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Identification of differentially expressed genes in papillary thyroid cancers

Authors
 Ki-Young Lee  ;  Song Mei Huang  ;  Shengjin Li  ;  Jin-Man Kim 
Citation
 YONSEI MEDICAL JOURNAL, Vol.50(1) : 60-67, 2009 
Journal Title
 YONSEI MEDICAL JOURNAL 
ISSN
 0513-5796 
Issue Date
2009
MeSH
Adaptor Proteins, Signal Transducing ; Adolescent ; Adult ; Aged ; Carcinoma, Papillary/genetics* ; Collagen Type I/genetics* ; Female ; Gene Expression Profiling* ; Gene Expression Regulation, Neoplastic* ; Humans ; Male ; Membrane Proteins/genetics* ; Middle Aged ; Reverse Transcriptase Polymerase Chain Reaction ; Thyroid Neoplasms/genetics* ; Young Adult
Keywords
Papillary thyroid cancer ; dendritic cell-specific transmembrane protein ; differentially expressed genes ; type I collagen A1
Abstract
PURPOSE: Techniques designed to identify differentially expressed genes (DEGs) in tumors have become important in modern pathology. Genefishing technique using the annealing control primer (ACP) system has recently been developed to screen for DEG transcripts. We tried to identify DEGs involved in papillary thyroid cancer (PTC) by using Genefishing technique. MATERIALS AND METHODS: We utilized a new differential display method, designated with Genefishing technique, to analyze DEGs in 21 cases of PTCs. RESULTS: Comparing the gene expression profiles between PTC and normal thyroid, we detected 17 genes that were differentially expressed in PTCs and performed cloning with sequencing in 10 genes. We confirmed the expression patterns of 2 DEGs by RT-PCR assay and identified the same results in 17 out of 21 (81%) PTCs. The 2 DEGs over-expressed in PTCs were identified as DC-STAMP and type I collagen A1. They are novel genes identified first in PTCs. CONCLUSION: We confirmed 2 DEGs in PTCs as DC-STAMP and type I collagen A1 by using Genefishing technique. Although the detailed functions of those 2 genes and their products remain to be determined, the genes will provide insights into mechanisms of carcinogenesis or tumor progression in PTCs.
Files in This Item:
T200900556.pdf Download
DOI
10.3349/ymj.2009.50.1.60
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anesthesiology and Pain Medicine (마취통증의학교실) > 1. Journal Papers
Yonsei Authors
Lee, Ki Young(이기영) ORCID logo https://orcid.org/0000-0003-4893-3195
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/103498
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