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Fabrication of a microarray using a combination of the large circular sense and antisense DNA

Authors
 Kyung-Oh Doh  ;  Yun-Han Lee  ;  Kil-Hwan Han  ;  Seok-Yong Uhm  ;  Jong-Pil Kim  ;  Yun-Ui Bae  ;  Jeong-Hoh Park  ;  Ik-Jae Moon  ;  Jong-Gu Park 
Citation
 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, Vol.25(1) : 113-120, 2010 
Journal Title
 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 
ISSN
 1107-3756 
Issue Date
2010
MeSH
Cell Line, Transformed ; Cell Line, Tumor ; Cell Proliferation ; DNA, Antisense/genetics* ; DNA, Complementary/genetics ; DNA, Single-Stranded/genetics* ; Gene Expression Profiling/economics ; Gene Expression Profiling/methods* ; Gene Expression Regulation, Neoplastic ; Humans ; Lung Neoplasms/genetics* ; Oligonucleotide Array Sequence Analysis/economics ; Oligonucleotide Array Sequence Analysis/methods* ; Reproducibility of Results ; Reverse Transcriptase Polymerase Chain Reaction
Abstract
In the present study, single-stranded large circular (LC)-sense molecules were utilized as probes for DNA microarrays and showed stronger binding signals than those of PCR-amplified cDNA probes. A microarray experiment using 284 LC-sense DNA probes found 6 upregulated and 7 downregulated genes in A549 cells as compared to WI38VA13 cells. Repeated experiments showed largely consistent results, and microarray data strongly correlated with data acquired from quantitative real-time RT-PCR. A large array comprising 5,079 LC-sense DNA was prepared, and analysis of the mean differential expression from dye-swap experiments revealed 332 upregulated and 509 downregulated genes in A549 cells compared to WI38VA13 cells. Subsequent functional analysis using an LC-antisense library of overexpressed genes identified 28 genes involved in A549 cell growth. These experiments demonstrated the proper features of LC-sense molecules as probe DNA for microarray and the potential utility of the combination of LC-sense and -antisense libraries for an effective functional validation of genes.
Full Text
http://www.spandidos-publications.com/ijmm/25/1/113
DOI
10.3892/ijmm_00000320
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers
Yonsei Authors
Lee, Yun Han(이윤한)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/103298
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