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Fabrication of a microarray using a combination of the large circular sense and antisense DNA
DC Field | Value | Language |
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dc.contributor.author | 이윤한 | - |
dc.date.accessioned | 2015-04-23T17:52:22Z | - |
dc.date.available | 2015-04-23T17:52:22Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 1107-3756 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/103298 | - |
dc.description.abstract | In the present study, single-stranded large circular (LC)-sense molecules were utilized as probes for DNA microarrays and showed stronger binding signals than those of PCR-amplified cDNA probes. A microarray experiment using 284 LC-sense DNA probes found 6 upregulated and 7 downregulated genes in A549 cells as compared to WI38VA13 cells. Repeated experiments showed largely consistent results, and microarray data strongly correlated with data acquired from quantitative real-time RT-PCR. A large array comprising 5,079 LC-sense DNA was prepared, and analysis of the mean differential expression from dye-swap experiments revealed 332 upregulated and 509 downregulated genes in A549 cells compared to WI38VA13 cells. Subsequent functional analysis using an LC-antisense library of overexpressed genes identified 28 genes involved in A549 cell growth. These experiments demonstrated the proper features of LC-sense molecules as probe DNA for microarray and the potential utility of the combination of LC-sense and -antisense libraries for an effective functional validation of genes. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 113~120 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Cell Line, Transformed | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Cell Proliferation | - |
dc.subject.MESH | DNA, Antisense/genetics* | - |
dc.subject.MESH | DNA, Complementary/genetics | - |
dc.subject.MESH | DNA, Single-Stranded/genetics* | - |
dc.subject.MESH | Gene Expression Profiling/economics | - |
dc.subject.MESH | Gene Expression Profiling/methods* | - |
dc.subject.MESH | Gene Expression Regulation, Neoplastic | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Lung Neoplasms/genetics* | - |
dc.subject.MESH | Oligonucleotide Array Sequence Analysis/economics | - |
dc.subject.MESH | Oligonucleotide Array Sequence Analysis/methods* | - |
dc.subject.MESH | Reproducibility of Results | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.title | Fabrication of a microarray using a combination of the large circular sense and antisense DNA | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Radiation Oncology (방사선종양학) | - |
dc.contributor.googleauthor | Kyung-Oh Doh | - |
dc.contributor.googleauthor | Yun-Han Lee | - |
dc.contributor.googleauthor | Kil-Hwan Han | - |
dc.contributor.googleauthor | Seok-Yong Uhm | - |
dc.contributor.googleauthor | Jong-Pil Kim | - |
dc.contributor.googleauthor | Yun-Ui Bae | - |
dc.contributor.googleauthor | Jeong-Hoh Park | - |
dc.contributor.googleauthor | Ik-Jae Moon | - |
dc.contributor.googleauthor | Jong-Gu Park | - |
dc.identifier.doi | 10.3892/ijmm_00000320 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03029 | - |
dc.relation.journalcode | J01132 | - |
dc.identifier.eissn | 1791-244X | - |
dc.identifier.pmid | 19956909 | - |
dc.identifier.url | http://www.spandidos-publications.com/ijmm/25/1/113 | - |
dc.contributor.alternativeName | Lee, Yun Han | - |
dc.contributor.affiliatedAuthor | Lee, Yun Han | - |
dc.citation.volume | 25 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 113 | - |
dc.citation.endPage | 120 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, Vol.25(1) : 113-120, 2010 | - |
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