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A promoter polymorphism -2122C>T of CHI3L1 is associated with serum low density lipoprotein cholesterol level in Korean subjects

Authors
 Haeyong Lee  ;  Ryunhwa Kang  ;  Sun Ha Jee  ;  Yoosik Yoon 
Citation
 CLINICAL BIOCHEMISTRY, Vol.43(15) : 1195-1200, 2010 
Journal Title
 CLINICAL BIOCHEMISTRY 
ISSN
 0009-9120 
Issue Date
2010
MeSH
Adipokines ; Asian Continental Ancestry Group/genetics* ; Base Pairing/genetics ; Chitinase-3-Like Protein 1 ; Cholesterol, LDL/blood* ; Female ; Gene Expression Regulation ; Genotype ; Glycoproteins/genetics* ; Glycoproteins/metabolism ; Humans ; Lectins/genetics* ; Lectins/metabolism ; Male ; Metabolome/genetics ; Middle Aged ; Nuclear Proteins/metabolism ; Polymorphism, Single Nucleotide/genetics* ; Promoter Regions, Genetic* ; Protein Binding ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Republic of Korea
Keywords
CHI3L1 ; Promoter ; SNP ; rs946261 ; LDL cholesterol ; EMSA
Abstract
OBJECTIVES: This study assessed the effects of 10 tagging single nucleotide polymorphisms (SNPs) of the CHI3L1 gene on serum LDL cholesterol levels in 290 Korean subjects. DESIGN AND METHODS: Genotyping analyses of SNPs were conducted by TaqMan® method. The effects of the promoter SNP on mRNA expression and nuclear factor binding were measured by real-time PCR and electrophoretic mobility shift assay, respectively. RESULTS: Among 10 tagging SNPs, -2122C>T SNP (rs946261) in the promoter region was significantly associated with serum LDL cholesterol level (P=0.005). The T allele of -2122C>T was associated with significantly increased mRNA expressions in peripheral blood cells of the subjects, and also increased a nuclear factor binding measured by an electrophoretic mobility shift assay. CONCLUSIONS: -2122C>T of CHI3L1, a promoter SNP which affects the mRNA expression and nuclear factor binding, is significantly associated with serum LDL cholesterol levels in Korean subjects.
Full Text
http://www.sciencedirect.com/science/article/pii/S0009912010003322
DOI
10.1016/j.clinbiochem.2010.07.017
Appears in Collections:
4. Graduate School of Public Health (보건대학원) > Graduate School of Public Health (보건대학원) > 1. Journal Papers
Yonsei Authors
Jee, Sun Ha(지선하) ORCID logo https://orcid.org/0000-0001-9519-3068
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/103193
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