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Preparation and Characterization of Genetically Engineered Mesenchymal Stem Cell Aggregates for Regenerative Medicine
DC Field | Value | Language |
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dc.contributor.author | 김선화 | - |
dc.contributor.author | 문형호 | - |
dc.contributor.author | 최동훈 | - |
dc.date.accessioned | 2015-04-23T17:44:55Z | - |
dc.date.available | 2015-04-23T17:44:55Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 2093-5552 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/103055 | - |
dc.description.abstract | ABSTRACT − Combining cell- and gene-based therapy is a promising therapeutic strategy in regenerative medicine. The aim of this study was to develop genetically modified mesenchymal stem cell (MSC) aggregates using a poly(ethylene glycol) (PEG) hydrogel micro-well array technique. Stable PEG hydrogel micro-well arrays with diameters of 200 to 500 μm were fabricated and used to generate genetically engineered MSC aggregates. Rat bone marrow-derived MSCs were transfected with a green fluorescent protein (GFP) plasmid as a reporter gene, and aggregated by culturing in the PEG hydrogel micro-well arrays. The resultant cell aggregates had a mean diameter of less than 200 μm, and maintained the mesenchymal phenotype even after genetic modification and cell aggregation. Transplantation of MSC aggregates that are genetically modified to express therapeutic or cell-survival genes may be a potential therapeutic approach for regenerative medicine | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 333~337 | - |
dc.relation.isPartOf | Journal of Pharmaceutical Investigation | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Preparation and Characterization of Genetically Engineered Mesenchymal Stem Cell Aggregates for Regenerative Medicine | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Sun Hwa Kim | - |
dc.contributor.googleauthor | Hyung-Ho Moon | - |
dc.contributor.googleauthor | Bong Genn Chung | - |
dc.contributor.googleauthor | Donghoon Choi | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00561 | - |
dc.contributor.localId | A01392 | - |
dc.contributor.localId | A04053 | - |
dc.relation.journalcode | J01699 | - |
dc.subject.keyword | Cell and gene therapy | - |
dc.subject.keyword | Mesenchymal stem cells | - |
dc.subject.keyword | Micro-well array | - |
dc.subject.keyword | Cell aggregates | - |
dc.contributor.alternativeName | Kim, Sun Hwa | - |
dc.contributor.alternativeName | Moon, Hyung Ho | - |
dc.contributor.alternativeName | Choi, Dong Hoon | - |
dc.contributor.affiliatedAuthor | Kim, Sun Hwa | - |
dc.contributor.affiliatedAuthor | Moon, Hyung Ho | - |
dc.contributor.affiliatedAuthor | Choi, Dong Hoon | - |
dc.citation.volume | 40 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 333 | - |
dc.citation.endPage | 337 | - |
dc.identifier.bibliographicCitation | Journal of Pharmaceutical Investigation, Vol.40(6) : 333-337, 2010 | - |
dc.identifier.rimsid | 35653 | - |
dc.type.rims | ART | - |
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