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Development and analysis of a collagen-based hemostatic adhesive

 Seung Hyuk Baik  ;  Jae Hyung Kim  ;  Hyun Hee Cho  ;  Si-Nae Park  ;  Yu Seun Kim  ;  Hwal Suh 
 JOURNAL OF SURGICAL RESEARCH, Vol.164(2) : 221-228, 2010 
Journal Title
Issue Date
Animals ; Blood Coagulation ; Collagen*/therapeutic use ; Drug Carriers ; Hemorrhage/prevention & control ; Hemostasis ; Hemostatics* ; Humans ; Liver/cytology ; Liver/surgery ; Liver Circulation ; Male ; Models, Animal ; Postoperative Complications/therapy ; Rats ; Rats, Sprague-Dawley ; Solutions ; Tensile Strength ; Tissue Adhesives*
hemostat ; adhesive ; atelocollagen ; aminocaproic acid ; tranexamic acid
BACKGROUND: Commercially available hemostatic-adhesive has the risk of disease transmission because it is derived from a plasma component. The purpose of this study is to manufacture a new hemostatic adhesive and evaluate its performances. METHODS: Two atelocollagen-based hemostatic adhesives were produced and named as Bleestop A (2% esterified atelocollagen in 75% ethanol + 1% CaCl(2) + 0.71% DOPA + 0.1% tranexamic acid in DW) and Bleestop B (2% esterified atelocollagen in DW + 1% CaCl(2) + 0.71% DOPA + 0.1% aminocaproic acid in DW). These are compared with the negative control group (no adhesives used group) and the positive control group (Tissucol Duo Quick group). The adult male Sprague-Dawley rat model was adopted and the hemostatic-adhesion activities of each group were assessed by the adhesion strength test and the morphologic features of adhesion. The liver tissues were used. Histologic assessment of adhesion was accessed using light microscopy. RESULTS: Bleeding was controlled immediately after application of Bleestop A and partial adhesion was observed after 30 s. More than 95% area of the resected surface attached after 1 min. In Bleestop B, partial adhesion was observed after 30 s. More than 95% area of the resected surface was attached after 45 s. Histologic evaluation showed that Bleestop A and B mediated organized adhesion rapidly between both resected tissue surfaces. The adhesion strength of Bleestop A and B was better than the negative control group and showed same adhesion strength as the positive control group after 3 min. CONCLUSION: Bleestop A and B showed significant hemostasis and adhesion capability within 1 min. They are comparable to the positive control group.
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1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Yu Seun(김유선) ORCID logo https://orcid.org/0000-0002-5105-1567
Kim, Jae Hyung(김재형)
Baik, Seung Hyuk(백승혁) ORCID logo https://orcid.org/0000-0003-4183-2332
Suh, Hwal(서활)
Cho, Hyun Hee(조현희)
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