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IgE binding epitopes of Bla g 6 from German cockroach

Authors
 Un  ;  Sunjin  ;  Yong Jeong  ;  Kyoung  ;  Yi  ;  Myung-hee  ;  Kim  ;  Chung-ryul  ;  Yong  ;  Tai-Soon 
Citation
 Protein and Peptide Letters, Vol.17(9) : 1170-1176, 2010 
Journal Title
 Protein and Peptide Letters 
ISSN
 0929-8665 
Issue Date
2010
MeSH
Adolescent ; Adult ; Allergens/chemistry ; Allergens/immunology ; Allergens/metabolism ; Animals ; Calcium-Binding Proteins/metabolism ; Child ; Cockroaches/immunology* ; Epitopes/chemistry ; Epitopes/immunology* ; Epitopes/metabolism* ; Female ; Humans ; Immunoglobulin E/immunology* ; Immunoglobulin E/metabolism* ; Male ; Middle Aged ; Troponin C/metabolism ; Young Adult
Keywords
Allergen ; Blattella germanica ; calcium binding protein ; IgE ; epitope ; troponin C
Abstract
Bla g 6, a German cockroach allergen, shows homology to muscle protein troponin C. It contains four calcium-binding domains at amino acid (aa) residues 20-30, 56-67, 96-107, and 132-143, and its immunoglobulin E (IgE) reactivity is dependent upon calcium ion level. However, the IgE binding epitopes of Bla g 6 have not been investigated. This study aimed to analyze the IgE binding epitopes from the five peptide fragments of Bla g 6. The full-length of three Bla g 6 isoallergens (Bla g 6.0101, Bla g 6.0201, and Bla g 6.0301) and five peptide fragments (P1: aa 1-111, P2: aa 1-95, P3: aa 33-111, P4: aa 80-151, and P5: aa 33-151) of Bla g 6.0101 were generated by polymerase chain reaction (PCR) and expressed in Escherichia coli. Enzyme-linked immunosorbent assay (ELISA) was performed on 24 patients' sera that adjusted the final concentration 10 mM of CaCl(2) to determine the IgE activities of Bla g 6. Eight sera (33.3%), 9 sera (37.5%), and 11 sera (45.8%) showed IgE reactivity to Bla g 6.0101, Bla g 6.0201, and Bla g 6.0301, respectively. Among the sera from the positive IgE reactivity, three patients' sera were selected and the IgE reactivity was measured by ELISA with the five peptide fragments of Bla g 6. Based on IgE responses, one patient's serum exhibited the strongest IgE reactivity. We assumed that the aa between 96-151 residues, including the calcium binding domains III and IV, would be important for IgE binding. These results may provide information that will yield safe diagnostic methods and immunotherapeutics
Full Text
http://www.ingentaconnect.com/content/ben/ppl/2010/00000017/00000009/art00016?token=005015f9131a041333c4a2f7a7a6a6f2c4067283e6646734f6d622244502c6876305021b871d54b2
DOI
10.2174/092986610791760432
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Institute of Allergy (알레르기연구소) > 1. Journal Papers
Yonsei Authors
Kim, Chung Ryul(김충렬)
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
Yi, Myung Hee(이명희) ORCID logo https://orcid.org/0000-0001-9537-5726
Jeong, Kyoung Yong(정경용) ORCID logo https://orcid.org/0000-0001-9887-1426
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/102737
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