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IgE binding epitopes of Bla g 6 from German cockroach

 Un  ;  Sunjin  ;  Yong Jeong  ;  Kyoung  ;  Yi  ;  Myung-hee  ;  Kim  ;  Chung-ryul  ;  Yong  ;  Tai-Soon 
 PROTEIN AND PEPTIDE LETTERS, Vol.17(9) : 1170-1176, 2010 
Journal Title
Issue Date
Adolescent ; Adult ; Allergens/chemistry ; Allergens/immunology ; Allergens/metabolism ; Animals ; Calcium-Binding Proteins/metabolism ; Child ; Cockroaches/immunology* ; Epitopes/chemistry ; Epitopes/immunology* ; Epitopes/metabolism* ; Female ; Humans ; Immunoglobulin E/immunology* ; Immunoglobulin E/metabolism* ; Male ; Middle Aged ; Troponin C/metabolism ; Young Adult
Allergen ; Blattella germanica ; calcium binding protein ; IgE ; epitope ; troponin C
Bla g 6, a German cockroach allergen, shows homology to muscle protein troponin C. It contains four calcium-binding domains at amino acid (aa) residues 20-30, 56-67, 96-107, and 132-143, and its immunoglobulin E (IgE) reactivity is dependent upon calcium ion level. However, the IgE binding epitopes of Bla g 6 have not been investigated. This study aimed to analyze the IgE binding epitopes from the five peptide fragments of Bla g 6. The full-length of three Bla g 6 isoallergens (Bla g 6.0101, Bla g 6.0201, and Bla g 6.0301) and five peptide fragments (P1: aa 1-111, P2: aa 1-95, P3: aa 33-111, P4: aa 80-151, and P5: aa 33-151) of Bla g 6.0101 were generated by polymerase chain reaction (PCR) and expressed in Escherichia coli. Enzyme-linked immunosorbent assay (ELISA) was performed on 24 patients' sera that adjusted the final concentration 10 mM of CaCl(2) to determine the IgE activities of Bla g 6. Eight sera (33.3%), 9 sera (37.5%), and 11 sera (45.8%) showed IgE reactivity to Bla g 6.0101, Bla g 6.0201, and Bla g 6.0301, respectively. Among the sera from the positive IgE reactivity, three patients' sera were selected and the IgE reactivity was measured by ELISA with the five peptide fragments of Bla g 6. Based on IgE responses, one patient's serum exhibited the strongest IgE reactivity. We assumed that the aa between 96-151 residues, including the calcium binding domains III and IV, would be important for IgE binding. These results may provide information that will yield safe diagnostic methods and immunotherapeutics
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1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Institute of Allergy (알레르기연구소) > 1. Journal Papers
Yonsei Authors
Kim, Chung Ryul(김충렬)
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
Yi, Myung Hee(이명희) ORCID logo https://orcid.org/0000-0001-9537-5726
Jeong, Kyoung Yong(정경용) ORCID logo https://orcid.org/0000-0001-9887-1426
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