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Enhanced production of red blood cells in suspension by electrostatic interactions with culture plates

Authors
 Eun Jung Baek  ;  Jungmok You  ;  Min Sun Kim  ;  So-Young Lee  ;  Seong-Je Cho  ;  Eunkyoung Kim  ;  Hyun Ok Kim 
Citation
 TISSUE ENGINEERING PART C-METHODS, Vol.16(6) : 1325-1334, 2010 
Journal Title
 TISSUE ENGINEERING PART C-METHODS 
ISSN
 1937-3384 
Issue Date
2010
MeSH
Cell Culture Techniques/instrumentation ; Cell Culture Techniques/methods ; Cell Differentiation/physiology* ; Cell Proliferation ; Coculture Techniques/methods ; Erythrocyte Count ; Erythrocytes/cytology* ; Erythrocytes/metabolism ; Erythrocytes/physiology* ; Fetal Blood/cytology ; Fetal Blood/physiology ; Humans ; Models, Biological ; Static Electricity* ; Surface Properties ; Suspensions ; Up-Regulation/physiology
Abstract
Enucleation of erythroblasts, a critical step in the generation of red blood cells (RBCs), occurs at a low rate without cocultured stromal cells. Previously, the surface properties of the cell culture plate were not considered in the enucleation process, because the cells exist in suspension. Here, we show that a significantly higher rate of enucleation of erythroblasts occurred on the positively charged plates than on the negatively charged surfaces or the both negatively and positively charged plates. Also, the negatively and positively charged plate group showed a significantly higher enucleation than did the hydrophobic plates. Therefore, the plates fully coated with amine groups generated 1.88 times more enucleated RBCs than did the hydrophobic plates. This study suggests an important insight into the effect of surface characteristics of cell culture plates on suspension cell culture. Further, this simple and inexpensive procedure could contribute to a more efficient RBC production system, eliminating the need for robust and expensive coculture procedures.
Full Text
http://online.liebertpub.com/doi/abs/10.1089/ten.TEC.2009.0785
DOI
10.1089/ten.tec.2009.0785
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyun Ok(김현옥) ORCID logo https://orcid.org/0000-0002-4964-1963
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/102639
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