Cited 10 times in
Enhanced production of red blood cells in suspension by electrostatic interactions with culture plates
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김현옥 | - |
dc.date.accessioned | 2015-04-23T17:31:43Z | - |
dc.date.available | 2015-04-23T17:31:43Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 1937-3384 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/102639 | - |
dc.description.abstract | Enucleation of erythroblasts, a critical step in the generation of red blood cells (RBCs), occurs at a low rate without cocultured stromal cells. Previously, the surface properties of the cell culture plate were not considered in the enucleation process, because the cells exist in suspension. Here, we show that a significantly higher rate of enucleation of erythroblasts occurred on the positively charged plates than on the negatively charged surfaces or the both negatively and positively charged plates. Also, the negatively and positively charged plate group showed a significantly higher enucleation than did the hydrophobic plates. Therefore, the plates fully coated with amine groups generated 1.88 times more enucleated RBCs than did the hydrophobic plates. This study suggests an important insight into the effect of surface characteristics of cell culture plates on suspension cell culture. Further, this simple and inexpensive procedure could contribute to a more efficient RBC production system, eliminating the need for robust and expensive coculture procedures. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1325~1334 | - |
dc.relation.isPartOf | TISSUE ENGINEERING PART C-METHODS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Cell Culture Techniques/instrumentation | - |
dc.subject.MESH | Cell Culture Techniques/methods | - |
dc.subject.MESH | Cell Differentiation/physiology* | - |
dc.subject.MESH | Cell Proliferation | - |
dc.subject.MESH | Coculture Techniques/methods | - |
dc.subject.MESH | Erythrocyte Count | - |
dc.subject.MESH | Erythrocytes/cytology* | - |
dc.subject.MESH | Erythrocytes/metabolism | - |
dc.subject.MESH | Erythrocytes/physiology* | - |
dc.subject.MESH | Fetal Blood/cytology | - |
dc.subject.MESH | Fetal Blood/physiology | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Models, Biological | - |
dc.subject.MESH | Static Electricity* | - |
dc.subject.MESH | Surface Properties | - |
dc.subject.MESH | Suspensions | - |
dc.subject.MESH | Up-Regulation/physiology | - |
dc.title | Enhanced production of red blood cells in suspension by electrostatic interactions with culture plates | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학) | - |
dc.contributor.googleauthor | Eun Jung Baek | - |
dc.contributor.googleauthor | Jungmok You | - |
dc.contributor.googleauthor | Min Sun Kim | - |
dc.contributor.googleauthor | So-Young Lee | - |
dc.contributor.googleauthor | Seong-Je Cho | - |
dc.contributor.googleauthor | Eunkyoung Kim | - |
dc.contributor.googleauthor | Hyun Ok Kim | - |
dc.identifier.doi | 10.1089/ten.tec.2009.0785 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01122 | - |
dc.relation.journalcode | J03051 | - |
dc.identifier.eissn | 1937-3392 | - |
dc.identifier.pmid | 20302446 | - |
dc.identifier.url | http://online.liebertpub.com/doi/abs/10.1089/ten.TEC.2009.0785 | - |
dc.contributor.alternativeName | Kim, Hyun Ok | - |
dc.contributor.affiliatedAuthor | Kim, Hyun Ok | - |
dc.citation.volume | 16 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 1325 | - |
dc.citation.endPage | 1334 | - |
dc.identifier.bibliographicCitation | TISSUE ENGINEERING PART C-METHODS, Vol.16(6) : 1325-1334, 2010 | - |
dc.identifier.rimsid | 55345 | - |
dc.type.rims | ART | - |
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