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Neuroprotective effect of combined hypoxia-induced VEGF and bone marrow-derived mesenchymal stem cell treatment

 Sung Su An  ;  Hong Lian Jin  ;  Keung Nyun Kim  ;  Hyun Ah Kim  ;  Dong Seok Kim  ;  Joon Cho  ;  Meng-Lu Liu  ;  Jin Soo Oh  ;  Do Heum Yoon  ;  Min Hyung Lee  ;  Yoon Ha 
 CHILDS NERVOUS SYSTEM, Vol.26(3) : 323-331, 2010 
Journal Title
Issue Date
Animals ; Apoptosis/physiology ; Bone Marrow Transplantation* ; Cell Hypoxia/physiology ; Cell Line, Tumor ; Cells, Cultured ; Coculture Techniques ; Genetic Vectors ; Male ; Mesenchymal Stem Cell Transplantation* ; Neurons/physiology* ; Rats ; Rats, Sprague-Dawley ; Transfection ; Vascular Endothelial Growth Factor A/genetics* ; Vascular Endothelial Growth Factor A/metabolism
PURPOSES: To avoid unwanted adverse effects of higher doses of single treatment of stem cells and gene therapy and increase the therapeutic efficacies, we hypothesized the combined therapy with stem cells and gene therapy. This study assessed the neuroprotective effects of combined gene therapy and stem cell treatment under ischemic hypoxia conditions using hypoxia-inducible vascular endothelial growth factor (VEGF) and bone marrow-derived mesenchymal stem cells (BMSC). METHODS: Experimental groups included the control which was N2A cells transfected with empty vectors, the transfection only group which was N2A cells treated with pEpo-SV-VEGF alone, the BMSC only group which was N2A cells transfected with empty vectors and cocultured with BMSCs, and the combined treatment group which was N2A cells treated with pEpo-SV-VEGF and cocultured with BMSCs. Each group was transfected for 4 h and cultured at 37 degrees C and 5% CO2 for 24 h. Each group was then cultivated under hypoxic conditions (1% O2) for 12 h. Neuroprotective effects were assessed by reverse transcription polymerase chain reaction, annexin V, and cytotoxicity assay. RESULTS: Neurons exposed to hypoxic conditions exhibited neuronal apoptosis. Compared to single treatments, the combined hypoxia-inducible VEGF and BMSC treatment demonstrated a significant increase in VEGF expression and decreased neuronal apoptosis. CONCLUSIONS: These results suggest that combined pEpo-SV-VEGF and BMSC treatment is effective in protecting neurons against hypoxic ischemic injury.
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1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Keung Nyun(김긍년)
Kim, Dong Seok(김동석)
Jin, Hong Lian(김홍련)
An, Sung Su(안성수)
Oh, Jin Soo(오진수)
Yoon, Do Heum(윤도흠) ORCID logo https://orcid.org/0000-0003-1452-5724
Ha, Yoon(하윤)
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