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Involvement of Src Family Tyrosine Kinase in Apoptosis of Human Neutrophils Induced by Protozoan Parasite Entamoeba histolytica

Authors
 Seobo Sim  ;  Jae-Ran Yu  ;  Young Ah Lee  ;  Myeong Heon Shin 
Citation
 KOREAN JOURNAL OF PARASITOLOGY, Vol.48(4) : 285-290, 2010 
Journal Title
 KOREAN JOURNAL OF PARASITOLOGY 
ISSN
 0023-4001 
Issue Date
2010
MeSH
Apoptosis* ; Cells, Cultured ; Entamoeba histolytica/immunology* ; Entamoeba histolytica/pathogenicity* ; GPI-Linked Proteins/metabolism ; Genistein/metabolism ; Humans ; Neutrophils/immunology* ; Protein Kinase Inhibitors/metabolism ; Pyrimidines/metabolism ; Reactive Oxygen Species/metabolism ; Receptors, IgG/metabolism ; src-Family Kinases/antagonists & inhibitors ; src-Family Kinases/metabolism*
Keywords
Entamoeba histolytica ; Src tyrosine kinase ; apoptosis ; neutrophil
Abstract
Tyrosine kinases are one of the most important regulators for intracellular signal transduction related to inflammatory responses. However, there are no reports describing the effects of tyrosine kinases on neutrophil apoptosis induced by Entamoeba histolytica. In this study, isolated human neutrophils from peripheral blood were incubated with live trophozoites in the presence or absence of tyrosine kinase inhibitors. Entamoeba-induced receptor shedding of CD16 and PS externalization in neutrophils were inhibited by pre-incubation of neutrophils with the broad-spectrum tyrosine kinase inhibitor genistein or the Src family kinase inhibitor PP2. Entamoeba-induced ROS production was also inhibited by genistein or PP2. Moreover, genistein and PP2 blocked the phosphorylation of ERK and p38 MAPK in neutrophils induced by E. histolytica. These results suggest that Src tyrosine kinases may participate in the signaling event for ROS-dependent activation of MAPKs during neutrophil apoptosis induced by E. histolytica
Files in This Item:
T201004045.pdf Download
DOI
10.3347/kjp.2010.48.4.285
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Shin, Myeong Heon(신명헌) ORCID logo https://orcid.org/0000-0001-8207-6110
Lee, Young Ah(이영아) ORCID logo https://orcid.org/0000-0002-0414-842X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/102587
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