Objective:Ekman’s pictures of facial affect (Ekamn and Frisen, 1976) have been extensively used in the current affective neuroscience researches. However, their reliability and validity have not been established in Korea. The purpose of this study is to assess the reliability and validity of Ekman’s pictures for Koreans. Method:Using the 70 Ekman’s pictures, Korean young adults were asked to judge the category of the facial expressions and rate the emotional intensity. We selected the pictures, whose inter-rater agreement on the recognition of emotional category is more than 70%. To test criterion validity, we assessed the correlations of emotion recognition accuracy and in tensity between selected Ekman’s pictures and 28 Japanese pictures of Japanese and Caucasian Facial Expressions of Emotion (JACFEE). Results:Ten happy, 6 disgust, 6 anger, 9 sad, 10 surprise and 4 fear pictures were found to have more than 70% inter-rater agreement. The recognition accuracy and intensity rating of selected 45 Ekman’s pictures were significantly correlated with those of 28 Japanese photos of JACFEE. Conclusion:The selected Ekman’s pictures seem to have acceptable inter-rater reliability and validity for Koreans. We expect the selected set of Ekman’s pictures could be applied to Koreans for the affective neuroscience research.