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Proteomic molecular portrait of interface zone in breast cancer

Authors
 Suki Kang  ;  Min Ju Kim  ;  HeeJung An  ;  Baek Gil Kim  ;  Yoon Pyo Choi  ;  Kyu Sub Kang  ;  Ming-Qing Gao  ;  Hangran Park  ;  Hyung Jin Na  ;  Hye Kyung Kim  ;  Hae Ree Yun  ;  Dong Su Kim  ;  Nam Hoon Cho 
Citation
 JOURNAL OF PROTEOME RESEARCH, Vol.9(11) : 5638-5645, 2010 
Journal Title
 JOURNAL OF PROTEOME RESEARCH 
ISSN
 1535-3893 
Issue Date
2010
MeSH
Biomarkers, Tumor/analysis ; Breast Neoplasms/chemistry* ; Breast Neoplasms/pathology* ; Breast Neoplasms/surgery ; Disease Progression ; Female ; Guanine Nucleotide Dissociation Inhibitors ; Humans ; Microfilament Proteins ; Neoplasm Metastasis ; Neoplasm Proteins/analysis* ; Nuclear Proteins ; Peptide Mapping ; Proteomics/methods ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Tumor Microenvironment ; Up-Regulation ; rho-Specific Guanine Nucleotide Dissociation Inhibitors
Keywords
2-DE ; CAPG ; CK8 ; Interface zone ; Rho GDI alpha ; stromal-epithelial cell interactions ; tumor margin ; WDR1
Abstract
Surgical tumor margins are intended to encompass residual tumor cells but may not always accurately delineate the boundary between tumor and normal tissue. Efforts to define tumor margins based on molecular analysis have achieved limited success. Furthermore, no clinical trials have addressed the scope of the tumor microenvironment. Here, we considered the tumor cell population and surrounding microenvironment in delineating tumor margins, classifying breast cancer into tumor and normal zones, and introducing the concept of an interface zone, the region between the invading tumor front and normal tissue, which develops during tumor invasion and metastasis through remodeling of the tumor microenvironment. Pathological signatures of invasion markers in tumor tissues are most dynamic within the invading tumor front. We compared protein profiles of tumor, normal, and interface zones using MALDI-MS. Proteins upregulated in the interface zone were identified by peptide mass fingerprinting and confirmed by database searching with chemically assisted MALDI-PSD spectra. Upregulation was confirmed for RhoGDIα, CAPG, WDR1, and CK8 by Western and immunohistochemical analyses. Our results demonstrate that the molecular profile of the interface zone is unique and suggest that upregulation of proteins here may be related to progression and metastasis of breast carcinomas
Full Text
http://pubs.acs.org/doi/abs/10.1021/pr1004532
DOI
10.1021/pr1004532
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Kang, Kyu Sub(강규섭)
Kang, Suki(강숙희) ORCID logo https://orcid.org/0000-0002-9957-3479
Gao, Ming Qing(고명청)
Kim, Baek Gil(김백길) ORCID logo https://orcid.org/0000-0001-6270-1433
Cho, Nam Hoon(조남훈) ORCID logo https://orcid.org/0000-0002-0045-6441
Choi, Yoon Pyo(최윤표)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/102530
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