402 466

Cited 89 times in

Stromal fibroblasts from the interface zone of human breast carcinomas induce an epithelial-mesenchymal transition-like state in breast cancer cells in vitro.

 Ming-Qing Gao  ;  Baek Gil Kim  ;  Suki Kang  ;  Yoon Pyo Choi  ;  Hangran Park  ;  Kyu Sub Kang  ;  Nam Hoon Cho 
 JOURNAL OF CELL SCIENCE, Vol.123(pt 20) : 3507-3514, 2010 
Journal Title
Issue Date
Apoptosis/physiology ; Blotting, Western ; Breast Neoplasms/metabolism ; Breast Neoplasms/pathology* ; Cadherins/metabolism ; Cell Line, Tumor ; Coculture Techniques ; Epithelial-Mesenchymal Transition/physiology* ; Female ; Fibroblasts/metabolism* ; Flow Cytometry ; Fluorescent Antibody Technique ; Humans ; Matrix Metalloproteinase 14/metabolism ; Tumor Cells, Cultured ; Vimentin/metabolism
Fibroblasts were extracted from tissue in tumor burden zones, distal normal zones and interface zones between tumor and normal tissue of human breast carcinomas, and the corresponding fibroblasts were designated as cancer-associated fibroblasts (CAFs), normal zone fibroblasts (NFs) and interface zone fibroblasts (INFs). The crosstalk between three types of fibroblasts and breast cancer cells was evaluated using an in vitro direct co-culture model. We found that INFs grew faster and expressed higher levels of fibroblast activation protein than did NFs and CAFs. Compared with CAFs and NFs, INFs grown with breast cancer cells were significantly more effective in inducing an epithelial-mesenchymal transition (EMT) in cancer cells, as indicated by induction of vimentin and N-cadherin and downregulation of E-cadherin. This EMT process was also accompanied by activation of extracellular signal-regulated kinase (ERK) and modulation of membrane-type 1 matrix metalloproteinase (MT1-MMP) expression. Additionally, INFs promoted breast cell migration to a larger extent compared with NFs and CAFs. Taken together, these findings indicate that INFs isolated from the tumor interface zone exhibited more robust biological modulatory activity than did NFs and CAFs isolated from normal and tumor zones of the same tumor tissue, suggesting that the interface zone of the tumor represents a dynamic region vital to tumor progression.
Files in This Item:
T201003547.pdf Download
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Kang, Kyu Sub(강규섭)
Kang, Suki(강숙희) ORCID logo https://orcid.org/0000-0002-9957-3479
Kim, Baek Gil(김백길) ORCID logo https://orcid.org/0000-0001-6270-1433
Cho, Nam Hoon(조남훈) ORCID logo https://orcid.org/0000-0002-0045-6441
Choi, Yoon Pyo(최윤표)
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.