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Underlying mechanism for suppression of vascular smooth muscle cells by green tea polyphenol EGCG released from biodegradable polymers for stent application

Authors
 Dong-Wook Han  ;  Duk-Young Jung  ;  Jong-Chul Park  ;  Han Hee Cho  ;  Suong-Hyu Hyon  ;  Dong Keun Han 
Citation
 JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, Vol.95(2) : 424-433, 2010 
Journal Title
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
ISSN
 1549-3296 
Issue Date
2010
MeSH
Animals ; Antioxidants/chemistry ; Antioxidants/metabolism ; Antioxidants/pharmacology* ; Aorta/anatomy & histology ; Apoptosis/drug effects ; Biocompatible Materials/chemistry ; Biocompatible Materials/metabolism ; Catechin/analogs & derivatives* ; Catechin/chemistry ; Catechin/metabolism ; Catechin/pharmacology ; Cell Adhesion ; Cell Cycle/drug effects ; Cell Proliferation ; Cells, Cultured ; Dogs ; Male ; Materials Testing ; Matrix Metalloproteinase 2/metabolism ; Molecular Structure ; Muscle, Smooth, Vascular/cytology* ; Myocytes, Smooth Muscle/cytology ; Myocytes, Smooth Muscle/drug effects* ; Myocytes, Smooth Muscle/physiology ; NF-kappa B/metabolism ; Polymers*/chemistry ; Polymers*/metabolism ; Proto-Oncogene Proteins c-akt/metabolism ; Stents*
Keywords
epigallocatechin‐3‐ O‐gallate ; poly(lactide‐ co‐ε‐caprolactone) ; vascular smooth muscle cells ; phosphorylated Akt ; phosphorylated nuclear factor‐κB
Abstract
Epigallocatechin-3-O-gallate (EGCG), the predominant catechin from tea, is known to exert a variety of cardiovascular beneficial effects by affecting the activity of receptor and signal transduction kinases. In this study, we investigated the suppressive effects of EGCG released from biodegradable poly(L-lactide-co-ε-caprolactone, PLCL) films on the proliferation, cell cycle progression and matrix metalloproteinase-2 (MMP-2) expression of vascular smooth muscle cells (VSMCs). The involvement of phosphorylated Akt (pAkt) and nuclear factor-κB (pNF-κB) as well as the internalization of EGCG into VSMCs was also examined as underlying mechanisms for EGCG-mediated VSMC inhibition. The proliferation of canine aortic SMCs (CASMCs) on EGCG-releasing PLCL (E-PLCL) was significantly inhibited. The culture of CASMCs on E-PLCL resulted in induction of cell cycle arrest at G(0)/G(1) phase and inactivation of pAkt, leading to subsequent apoptosis. Active MMP-2 expression was directly lowered by EGCG released from E-PLCL and indirectly inhibited by the EGCG-mediated suppression of pNF-κB. We also observed the incorporation of fluorescein isothiocyanate-conjugated EGCG into the cytoplasm of CASMCs and its further nuclear translocation, which could lead to the interruption of the exogenous signals directed to genes responsible for cellular responses of CASMCs. Taken together, the attenuated responses of VSMCs to E-PLCL were shown to be mediated through the suppression of pNF-κB, pAkt and each subsequent target genes or proteins by EGCG incorporated into the cells
Full Text
http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.32870/abstract
DOI
10.1002/jbm.a.32870
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/102311
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