Cited 2 times in
Transduction of human EPO into human bone marrow mesenchymal stromal cells synergistically enhances cell-protective and migratory effects
DC Field | Value | Language |
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dc.contributor.author | 허용민 | - |
dc.date.accessioned | 2015-04-23T17:20:20Z | - |
dc.date.available | 2015-04-23T17:20:20Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0026-8933 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/102273 | - |
dc.description.abstract | Human bone marrow mesenchymal stromal cells (hBM-MSCs) are a promising tools for cell therapy. However, the poor viability of the transplanted cells is a major limiting factor. Human erythropoietin (hEPO) has been extensively studied in non-hematopoietic tissues for its neurotrophic, anti-oxidant, anti-apoptotic, and antiinflammatory effects. In this study, we evaluate whether transduction of the hEPO gene into MSCs provides protection and affects their migration. hBM-MSCs transduced with the hEPO gene (EPO-MSCs) stably secreted high levels of hEPO (10 IU/ml) with no alteration of their mesenchymal phenotype. MSCs were also treated with 10 IU rhEPO, an amount similar to what was secreted by EPO-MSCs, to generate 10U-MSCs. Protection against H2O2-induced oxidative stress and staurosporine-induced apoptosis was registered for both EPO-MSCs and 10U-MSCs, but the protective effects were higher for the EPO-MSCs than for the 10U-MSCs. EPO-MSCs had significantly higher migration rates compared to MSCs and 10U-MSCs. We confirmed that the intracellular signaling of ERK1/2 was higher in the EPO-MSCs than 10U-MSCs. This data demonstrates that the endogenous expression of EPO may efficiently initiate the ERK1/2 signaling pathway, resulting in synergistic effects on the production of neurotrophic factors. Thus, EPO-MSCs are a good candidate for cell therapy in ischemic and neurodegenerative diseases | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 656~663 | - |
dc.relation.isPartOf | MOLECULAR BIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Apoptosis/drug effects | - |
dc.subject.MESH | Apoptosis/physiology | - |
dc.subject.MESH | Bone Marrow Cells/cytology | - |
dc.subject.MESH | Bone Marrow Cells/metabolism* | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Cell Movement/drug effects | - |
dc.subject.MESH | Cell Movement/physiology* | - |
dc.subject.MESH | Enzyme Inhibitors/pharmacology | - |
dc.subject.MESH | Erythropoietin/biosynthesis* | - |
dc.subject.MESH | Erythropoietin/genetics | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Hydrogen Peroxide/pharmacology | - |
dc.subject.MESH | MAP Kinase Signaling System/drug effects | - |
dc.subject.MESH | MAP Kinase Signaling System/physiology* | - |
dc.subject.MESH | Mitogen-Activated Protein Kinase 1/genetics | - |
dc.subject.MESH | Mitogen-Activated Protein Kinase 1/metabolism | - |
dc.subject.MESH | Mitogen-Activated Protein Kinase 3/genetics | - |
dc.subject.MESH | Mitogen-Activated Protein Kinase 3/metabolism | - |
dc.subject.MESH | Oxidants/pharmacology | - |
dc.subject.MESH | Oxidative Stress/drug effects | - |
dc.subject.MESH | Oxidative Stress/physiology | - |
dc.subject.MESH | Staurosporine/pharmacology | - |
dc.subject.MESH | Stromal Cells/cytology | - |
dc.subject.MESH | Stromal Cells/metabolism | - |
dc.subject.MESH | Transduction, Genetic* | - |
dc.title | Transduction of human EPO into human bone marrow mesenchymal stromal cells synergistically enhances cell-protective and migratory effects | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Radiology (영상의학) | - |
dc.contributor.googleauthor | Mi-Hwa Kim | - |
dc.contributor.googleauthor | Goang-Won Cho | - |
dc.contributor.googleauthor | Seong-Ho Koh | - |
dc.contributor.googleauthor | Yong-Min Huh | - |
dc.contributor.googleauthor | Seung Hyun Kim | - |
dc.identifier.doi | 10.1134/S0026893310040126 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A04359 | - |
dc.relation.journalcode | J03199 | - |
dc.identifier.eissn | 1608-3245 | - |
dc.identifier.pmid | 20873225 | - |
dc.identifier.url | http://link.springer.com/article/10.1134%2FS0026893310040126 | - |
dc.contributor.alternativeName | Huh, Yong Min | - |
dc.contributor.affiliatedAuthor | Huh, Yong Min | - |
dc.citation.volume | 44 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 656 | - |
dc.citation.endPage | 663 | - |
dc.identifier.bibliographicCitation | MOLECULAR BIOLOGY, Vol.44(4) : 656-663, 2010 | - |
dc.identifier.rimsid | 51603 | - |
dc.type.rims | ART | - |
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