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Improved yield and functional parameters of rat pancreas islets isolated under intramuscular anesthesia

Authors
 Kim, Joon Ye  ;  Lee, Jeong-Ik  ;  Jeong, Jin Ho  ;  Fang, Yuhui  ;  Ju, Man Ki  ;  Kim, Soo Jin  ;  Huh, Kyu Ha  ;  Kim, Myoung Soo  ;  Kim, Yu Seun 
Citation
 Cell Transplantation, Vol.19(6) : 743-750, 2010 
Journal Title
 Cell Transplantation 
ISSN
 0963-6897 
Issue Date
2010
MeSH
Anesthesia* ; Anesthetics/administration & dosage ; Anesthetics/pharmacology ; Animals ; Cell Line ; Cell Survival/drug effects ; Enzyme-Linked Immunosorbent Assay ; Gene Expression Regulation/drug effects ; Glucose/pharmacology ; Injections, Intramuscular ; Insulin/genetics ; Insulin/secretion ; Islets of Langerhans/drug effects ; Islets of Langerhans/metabolism* ; Male ; Organ Preservation/methods* ; Pain/drug therapy ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Rats ; Rats, Inbred Lew ; Time Factors
Keywords
Rat islet ; RIN-5F ; Intraperitoneal anesthesia ; Intramuscular anesthesia
Abstract
Intraperitoneal (IP) anesthesia is commonly used for laboratory animal experiments including rat islet isolation. However, the direct effects of anesthetics on pancreatic islets have been neglected. This study compared the islet function and recovery yield from rats that were anesthetized using IP and intramuscular (IM) injection. In addition, the lag time required to lose deep pain was measured in the following anesthetics combinations. Lewis rats were anesthetized using ketamine and xylazine (K/X) or zoletil and xylazine (Z/X). A glucose challenge test was performed on each group of prepared islets. The effect of the anesthetic agents (e.g., ketamine, zoletil, xylazine alone, and the combination of K/X and Z/X) on cell lines (rat insulinoma; RIN-5F) was investigated by determining their effect on the cell viability, the amount of insulin, and insulin mRNA expression levels of RIN-5F. The time needed for deep anesthesia in IM anesthesia was significantly shortened in comparison to IP [K/X (IM: 313 ± 66 s, IP: 371 ± 84 s) and Z/X (IM: 206 ± 76 s, IP: 245 ± 92 s)]. In addition, number of isolated islet yield by IM anesthesia was significantly improved [K/X (IM: 1530 ± 242, IP: 1245 ± 149) and Z/X (IM: 1136 ± 226, IP: 511 ± 154)]. The functions of fresh islets, indicated by the stimulation index, acquired under IM anesthesia was better preserved than that of IP. The viability and the insulin secretion of RIN-5F were decreased at 24 and 48 h. Insulin gene expression levels were decreased at 24 h as well. Anesthetics may be absorbed through the pancreas surface to the islets and have a direct effect, resulting in islet exposure and deterioration during isolation. In conclusion, for rodent islet isolation, IM anesthesia is simpler and safer in comparison to IP anesthesia
Full Text
http://www.ingentaconnect.com/content/cog/ct/2010/00000019/F0020006/art00012
DOI
10.3727/096368910X508843
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kim, Myoung Soo(김명수) ORCID logo https://orcid.org/0000-0002-8975-8381
Kim, Soo Jin(김수진)
Kim, Yu Seun(김유선) ORCID logo https://orcid.org/0000-0002-5105-1567
Kim, Joon Ye(김준예)
Fang, Yu Hui(방우휘)
Jeong, Jin Ho(정진호)
Joo, Man Ki(주만기) ORCID logo https://orcid.org/0000-0002-4112-7003
Huh, Kyu Ha(허규하) ORCID logo https://orcid.org/0000-0003-1364-6989
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/102238
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