In this study, we aimed to develope the physio-module for echocardiography. This physio-module includes multi-functions such as ECG, respiration, PCG, heart sound, and this is used to diagnose a cardiac disease in using ultrasound images synchronized with biosignals of physio-module. In this paper, the developed physio-module was verified by applying various test patterns considering each biosignal's characteristics and we could get the performance of QRS trigger delay time within international standard, EC-13 criteria. And ECG's change in physio-module and blood flow in M-mode was synchronized