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고압-저온 보관에 따른 쥐 치아 치주인대세포의 활성도 평가
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김의성 | - |
dc.contributor.author | 김진 | - |
dc.contributor.author | 이승종 | - |
dc.contributor.author | 최성호 | - |
dc.date.accessioned | 2015-04-23T17:14:39Z | - |
dc.date.available | 2015-04-23T17:14:39Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 1225-0864 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/102094 | - |
dc.description.abstract | The purpose of this study was to evaluate the viability of periodontal ligament cells of rat teeth after low-temperature preservation under high pressure by means of MTT assay, WST-1 assay. 12 teeth of Sprague-Dawley white female rats of 4 week-old were used for each group. Both side of the first and second maxillary molars were extracted as atraumatically as possible under tiletamine anesthesia. The experimental groups were group 1 (Immediate extraction), group 2 (Slow freezing under pressure of 3 MPa), group 3 (Slow freezing under pressure of 2 MPa), group 4 (Slow freezing under no additional pressure), group 5 (Rapid freezing in liquid nitrogen under pressure of 2 MPa), group 6 (Rapid freezing in liquid nitrogen under no additional pressure), group 7 (low-temperature preservation at 0℃ under pressure of 2 MPa), group 8 (low-temperature preservation at 0℃ under no additional pressure), group 9 (low-temperature preservation at -5℃ under pressure of 90 MPa). F-medium and 10% DMSO were used as preservation medium and cryo-protectant. For cryo-preservation groups, thawing was performed in $37^{\circ}C$ water bath, then MTT assay, WST-1 assay were processed. One way ANOVA and Tukey HSD method were performed at the 95% level of confidence. The values of optical density obtained by MTT assay and WST-1 were divided by the values of eosin staining for tissue volume standardization. In both MTT and WST-1 assay, group 7 (0℃/2 MPa) showed higher viability of periodontal ligament cells than other group (2-6, 8) and this was statistically significant (p < 0.05), but showed lower viability than group 1, immediate extraction group (no statistical significance). By the results of this study, low-temperature preservation at 0℃ under pressure of 2 MPa suggest the possibility for long term preservation of teeth | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 285~294 | - |
dc.relation.isPartOf | Journal of Korean Academy of Operative Dentistry (대한치과보존학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | 고압-저온 보관에 따른 쥐 치아 치주인대세포의 활성도 평가 | - |
dc.title.alternative | THE EVALUATION OF PERIODONTAL LIGAMENT CELLS OF RAT TEETH AFTER LOW-TEMPERATURE PRESERVATION UNDER HIGH PRESSURE | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral Pathology (구강병리학) | - |
dc.contributor.googleauthor | 정진호 | - |
dc.contributor.googleauthor | 김 진 | - |
dc.contributor.googleauthor | 최성호 | - |
dc.contributor.googleauthor | 김의성 | - |
dc.contributor.googleauthor | 박지용 | - |
dc.contributor.googleauthor | 이승종 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00833 | - |
dc.contributor.localId | A02925 | - |
dc.contributor.localId | A01009 | - |
dc.contributor.localId | A04081 | - |
dc.relation.journalcode | J01488 | - |
dc.subject.keyword | Periodontal ligament cell | - |
dc.subject.keyword | MTT | - |
dc.subject.keyword | WST-1 | - |
dc.subject.keyword | Viability | - |
dc.subject.keyword | Low temperature preservation under pressure | - |
dc.subject.keyword | Rapid freezing | - |
dc.subject.keyword | Cryopreservation | - |
dc.contributor.alternativeName | Kim, Eui Seong | - |
dc.contributor.alternativeName | Kim, Jin | - |
dc.contributor.alternativeName | Lee, Seung Jong | - |
dc.contributor.alternativeName | Choi, Seong Ho | - |
dc.contributor.affiliatedAuthor | Kim, Eui Seong | - |
dc.contributor.affiliatedAuthor | Lee, Seung Jong | - |
dc.contributor.affiliatedAuthor | Kim, Jin | - |
dc.contributor.affiliatedAuthor | Choi, Seong Ho | - |
dc.citation.volume | 35 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 285 | - |
dc.citation.endPage | 294 | - |
dc.identifier.bibliographicCitation | Journal of Korean Academy of Operative Dentistry (대한치과보존학회지), Vol.35(4) : 285-294, 2010 | - |
dc.identifier.rimsid | 52719 | - |
dc.type.rims | ART | - |
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