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Comparative Analysis of Screening Results from Various ELISA Formats Used for Detection of Anti-Erythropoietin Antibodies in Korean Patients

Authors
 Sung Kyu HA  ;  Seung-Ju YANG  ;  Sug Kyun SHIN  ;  Young-Il JO  ;  Kyung-Min BAEK  ;  Seung-Hwa HONG  ;  Seung Pil PACK  ;  Sung-Jo KIM  ;  Tae-Hwe HEO 
Citation
 BIOMOLECULES & THERAPEUTICS, Vol.18(2) : 184-190, 2010 
Journal Title
 BIOMOLECULES & THERAPEUTICS 
ISSN
 1976-9148 
Issue Date
2010
Keywords
Erythropoietin ; Pure red-cell aplasia ; Bridging ELISA ; Immunogenicity
Abstract
Clinical cases of pure red cell aplasia (PRCA) have been reported during the recombinant human erythropoietin (EPO) therapy for the anemia patients. PRCA is a rare hematological disorder leading to a severe anemia due to an almost complete stop of red blood cell production. Antibody (Ab)-associated PRCA is caused by the EPO-neutralizing Abs that eliminate the biological activity of EPO. In order to detect anti-EPO Abs in human sera, we performed conventional ELISA, directly coated bridging ELISA, and streptavidin coated bridging ELISA, and compared their sensitivity and specificity. Some false positive results were obtained in the conventional ELISA. One positive sample was detected successfully by streptavidin coated bridging ELISA, which was not appeared in the directly coated bridging ELISA. In conclusion, streptavidin coated bridging ELISA was substantially sensitive and specific format and one out of sixty-eight serum samples was proved to be anti-EPO positive.
Files in This Item:
T201003269.pdf Download
DOI
10.4062/biomolther.2010.18.2.184
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Ha, Sung Kyu(하성규)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/102058
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