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Identification of streptococcal proteins reacting with sera from Behçet's disease and rheumatic disorders

Authors
 S. Cho  ;  J. Lee  ;  K. Ahn  ;  S. Cho  ;  Y. Park  ;  S. Lee  ;  D. Bang  ;  K. Lee 
Citation
 Clinical and Experimental Rheumatology, Vol.28(4 suppl 60) : 31-38, 2010 
Journal Title
 Clinical and Experimental Rheumatology 
ISSN
 0392-856X 
Issue Date
2010
Abstract
OBJECTIVES: We evaluated the reactivity of sera from Behçet's disease (BD), systemic lupus erythematosus (SLE), dermatomyositis (DM), rheumatoid arthritis (RA), and Takayasu's arteritis (TA) patients against human α-enolase and streptococcal α-enolase, and identified additional streptococcal antigens. METHODS: Enzyme-linked immunosorbent assay (ELISA) and immunoblotting were performed using sera from patients with BD, SLE, DM, RA, and TA and healthy volunteers (control) against human α-enolase and streptococcal α-enolase. Immunoblot analysis and matrix-assisted laser desorption ionisation-time-of-flight mass spectrometry were used to identify and recombine other streptococcal antigens. RESULTS: Specific positive signals against recombinant human α-enolase were detected by IgM ELISA of serum samples from 50% of BD, 14.3% of SLE, 57.1% of DM, 42.9% of RA, and 57.1% of TA patients. Specific positive signals against streptococcal α-enolase were detected from 42.9% of BD, 14.3% of DM, and 14.3% of TA patients. No SLE and RA sera reacted against streptococcal α-enolase antigen. Streptococcal proteins reacting with sera were identified as hypothetical protein (HP) for SLE and DM patients, acid phosphatase (AP) for RA patients, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) for TA patients. CONCLUSIONS: We observed that RA patients did not present serum reactivity against either HP or GAPDH though BD, SLE, DM, and TA patients did. Also, AP reacted with sera from BD, SLE, DM, RA, and TA patients.
Full Text
http://www.clinexprheumatol.org/abstract.asp?a=960
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실)
Yonsei Authors
박용범(Park, Yong Beom)
방동식(Bang, Dong Sik)
안근재(Ahn, Keun Jae)
이광훈(Lee, Kwang Hoon)
이수곤(Lee, Soo Kon)
이주희(Lee, Ju Hee) ORCID logo https://orcid.org/0000-0002-1739-5956
조성빈(Cho, Sung Bin)
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/101869
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