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Recombinant human epidermal growth factor (rhEGF) protects radiation-induced intestine injury in murine system

Authors
 Haejin OH  ;  Jinsil SEONG  ;  Wonwoo KIM  ;  Sooyeon PARK  ;  Woong Sub KOOM  ;  Nam Hoon CHO  ;  Mihee SONG 
Citation
 Journal of Radiation Research, Vol.51(5) : 535-541, 2010 
Journal Title
 Journal of Radiation Research 
ISSN
 0449-3060 
Issue Date
2010
MeSH
Animals ; Epidermal Growth Factor/genetics ; Epidermal Growth Factor/pharmacology* ; Humans ; Intestine, Small/drug effects* ; Intestine, Small/radiation effects* ; Male ; Mice ; Radiation Injuries/prevention & control* ; Radiation-Protective Agents/pharmacology* ; Recombinant Proteins/genetics ; Recombinant Proteins/pharmacology*
Abstract
This study was to investigate whether rhEGF protects radiation induced intestine injury without compromising antitumor effect of radiation in murine system. A radiation induced intestinal injury model was established in mice by whole body irradiation. Using this model, 4 groups were set; control, rhEGF (100 µg/kg intraperitoneally), radiation (10 Gy), and a combination (rhEGF and radiation). The level of apoptosis and proliferation were analyzed by TUNEL assay and proliferation cell nuclear antigen (PCNA) immunohistochemical staining, respectively, as well as observation of survival and body weight change. A tumor growth delay assay was performed using murine syngeneic tumors; one radioresistant tumor, HCa-I and one radiosensitive tumor, MCa-K. In the radiation induced intestinal injury model, the 10 Gy group had significantly more weight loss with less number of crypt cells and higher apoptosis than the 8 Gy group. Using 10 Gy model, radioprotective effect of rhEGF was tested. Addition of rhEGF improved not only the body weight loss but also survival following radiation. It also induced suppression of apoptosis as well as increase of PCNA expression and recovery of villi. rhEGF did not enhance the tumor growth after radiation exposure in the tested tumors. These findings suggest that combination of exogenous rhEGF and radiation can be a new anticancer strategy by protecting radiation-induced intestinal injury without alleviating antitumor effect of radiation
Files in This Item:
T201002903.pdf Download
DOI
10.1269/jrr.09145
Appears in Collections:
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Koom, Woong Sub(금웅섭) ORCID logo https://orcid.org/0000-0002-9435-7750
Kim, Won Woo(김원우)
Park, Soo Yeon(박수연)
Seong, Jin Sil(성진실) ORCID logo https://orcid.org/0000-0003-1794-5951
Oh, Hae Jin(오해진)
Cho, Nam Hoon(조남훈) ORCID logo https://orcid.org/0000-0002-0045-6441
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/101867
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