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Recombinant human epidermal growth factor (rhEGF) protects radiation-induced intestine injury in murine system

Authors
 Haejin OH  ;  Jinsil SEONG  ;  Wonwoo KIM  ;  Sooyeon PARK  ;  Woong Sub KOOM  ;  Nam Hoon CHO  ;  Mihee SONG 
Citation
 Journal of Radiation Research, Vol.51(5) : 535-541, 2010 
Journal Title
 Journal of Radiation Research 
ISSN
 0449-3060 
Issue Date
2010
Abstract
This study was to investigate whether rhEGF protects radiation induced intestine injury without compromising antitumor effect of radiation in murine system. A radiation induced intestinal injury model was established in mice by whole body irradiation. Using this model, 4 groups were set; control, rhEGF (100 µg/kg intraperitoneally), radiation (10 Gy), and a combination (rhEGF and radiation). The level of apoptosis and proliferation were analyzed by TUNEL assay and proliferation cell nuclear antigen (PCNA) immunohistochemical staining, respectively, as well as observation of survival and body weight change. A tumor growth delay assay was performed using murine syngeneic tumors; one radioresistant tumor, HCa-I and one radiosensitive tumor, MCa-K. In the radiation induced intestinal injury model, the 10 Gy group had significantly more weight loss with less number of crypt cells and higher apoptosis than the 8 Gy group. Using 10 Gy model, radioprotective effect of rhEGF was tested. Addition of rhEGF improved not only the body weight loss but also survival following radiation. It also induced suppression of apoptosis as well as increase of PCNA expression and recovery of villi. rhEGF did not enhance the tumor growth after radiation exposure in the tested tumors. These findings suggest that combination of exogenous rhEGF and radiation can be a new anticancer strategy by protecting radiation-induced intestinal injury without alleviating antitumor effect of radiation
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DOI
10.1269/jrr.09145
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실)
Yonsei Authors
금웅섭(Koom, Woong Sub) ORCID logo https://orcid.org/0000-0002-9435-7750
김원우(Kim, Won Woo)
박수연(Park, Soo Yeon)
성진실(Seong, Jin Sil) ORCID logo https://orcid.org/0000-0003-1794-5951
오해진(Oh, Hae Jin)
조남훈(Cho, Nam Hoon) ORCID logo https://orcid.org/0000-0002-0045-6441
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/101867
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