Cited 17 times in
Effects of the knockdown of hypoxia inducible factor-1α expression by adenovirus-mediated shRNA on angiogenesis and tumor growth in hepatocellular carcinoma cell lines
DC Field | Value | Language |
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dc.contributor.author | 김도영 | - |
dc.contributor.author | 노원상 | - |
dc.contributor.author | 박준용 | - |
dc.contributor.author | 신혜원 | - |
dc.contributor.author | 유지영 | - |
dc.contributor.author | 윤채옥 | - |
dc.contributor.author | 최성훈 | - |
dc.contributor.author | 한광협 | - |
dc.date.accessioned | 2015-04-23T17:07:01Z | - |
dc.date.available | 2015-04-23T17:07:01Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 1226-0479 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/101851 | - |
dc.description.abstract | BACKGROUND/AIMS: Hypoxia-inducible factor-1α (HIF-1α) is a central transcriptional factor involved in the cellular responses related to various aspects of cancer biology, including proliferation, survival, and angiogenesis, and the metabolism of the extracellular matrix in hypoxia. This study evaluated whether adenovirus-mediated small hairpin RNA (shRNA) against HIF-1α (shHIF-1α) inhibits cell proliferation and angiogenesis in hepatocellular carcinoma (HCC) cell lines. METHODS: Knockdown of HIF-1α expression was constructed by adenovirus-mediated RNA interference tools, and HCC cell lines infected with shHIF-1α coding virus were cultured under a hypoxia condition (1% O2) for 24 hours. Following infection, the expression levels of HIF-1α, angiogenesis factors, and matrix metalloproteinase (MMP) were examined using Western blotting. Cell proliferation and angiogenesis were measured by a cell proliferation assay (MTT assay) and an angiogenesis-related assay (invasion and tube-formation assay), respectively. RESULTS: Adenovirus mediated inhibition of HIF-1α induced suppression of tumor growth in HCC cell lines. It also down-regulated the expression of angiogenesis factor and MMP proteins. Angiogenesis as well as mobility of vascular cells to tumor was suppressed by adenovirus-mediated shHIF-1α-infected groups in human umbilical vein endothelial cells (HUVECs). CONCLUSIONS: These data suggest that adenovirus-mediated inhibition of HIF-1α inhibits the invasion, tube formation, and cell growth in HUVECs and HCC cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 280~287 | - |
dc.relation.isPartOf | Korean Journal of Hepatology (대한간학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adenoviridae/genetics | - |
dc.subject.MESH | Carcinoma, Hepatocellular/blood supply* | - |
dc.subject.MESH | Carcinoma, Hepatocellular/metabolism | - |
dc.subject.MESH | Carcinoma, Hepatocellular/therapy | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Cell Proliferation | - |
dc.subject.MESH | Endothelial Cells/metabolism | - |
dc.subject.MESH | Gene Knockdown Techniques | - |
dc.subject.MESH | Genetic Vectors | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Hypoxia-Inducible Factor 1, alpha Subunit/antagonists & inhibitors* | - |
dc.subject.MESH | Hypoxia-Inducible Factor 1, alpha Subunit/genetics | - |
dc.subject.MESH | Hypoxia-Inducible Factor 1, alpha Subunit/metabolism | - |
dc.subject.MESH | Liver Neoplasms/blood supply* | - |
dc.subject.MESH | Liver Neoplasms/metabolism | - |
dc.subject.MESH | Liver Neoplasms/therapy | - |
dc.subject.MESH | Matrix Metalloproteinases/metabolism | - |
dc.subject.MESH | Neovascularization, Pathologic/genetics | - |
dc.subject.MESH | Neovascularization, Pathologic/metabolism | - |
dc.subject.MESH | Neovascularization, Pathologic/therapy* | - |
dc.subject.MESH | RNA Interference | - |
dc.subject.MESH | RNA, Small Interfering/metabolism | - |
dc.title | Effects of the knockdown of hypoxia inducible factor-1α expression by adenovirus-mediated shRNA on angiogenesis and tumor growth in hepatocellular carcinoma cell lines | - |
dc.type | Article | - |
dc.contributor.college | Researcher Institutes (부설 연구소) | - |
dc.contributor.department | Liver Cirrhosis Clinical Research Center (간경변증임상연구센터) | - |
dc.contributor.googleauthor | Sung Hoon Choi | - |
dc.contributor.googleauthor | Hye Won Shin | - |
dc.contributor.googleauthor | Jun Yong Park | - |
dc.contributor.googleauthor | Ji Young Yoo | - |
dc.contributor.googleauthor | Do Young Kim | - |
dc.contributor.googleauthor | Weon Sang Ro | - |
dc.contributor.googleauthor | Chae-Ok Yun | - |
dc.contributor.googleauthor | Kwang-Hyub Han | - |
dc.identifier.doi | 10.3350/kjhep.2010.16.3.280 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01675 | - |
dc.contributor.localId | A02516 | - |
dc.contributor.localId | A02614 | - |
dc.contributor.localId | A04268 | - |
dc.contributor.localId | A04086 | - |
dc.contributor.localId | A02184 | - |
dc.contributor.localId | A00385 | - |
dc.contributor.localId | A01286 | - |
dc.relation.journalcode | J02036 | - |
dc.identifier.pmid | 20924210 | - |
dc.subject.keyword | Angiogenesis | - |
dc.subject.keyword | Hypoxia-inducible factor-1α (HIF-1α) | - |
dc.subject.keyword | Small hairpin RNA (shRNA) | - |
dc.subject.keyword | Human umbilical vein endothelial cells (HUVECs) | - |
dc.subject.keyword | Hepatocellular carcinoma | - |
dc.contributor.alternativeName | Kim, Do Young | - |
dc.contributor.alternativeName | Ro, Simon Weonsang | - |
dc.contributor.alternativeName | Park, Jun Yong | - |
dc.contributor.alternativeName | Shin, Hye Won | - |
dc.contributor.alternativeName | Yoo, Ji Yeong | - |
dc.contributor.alternativeName | Yun, Chae Ok | - |
dc.contributor.alternativeName | Choi, Sung Hoon | - |
dc.contributor.alternativeName | Han, Kwang Hyup | - |
dc.contributor.affiliatedAuthor | Park, Jun Yong | - |
dc.contributor.affiliatedAuthor | Yoo, Ji Yeong | - |
dc.contributor.affiliatedAuthor | Yun, Chae Ok | - |
dc.contributor.affiliatedAuthor | Han, Kwang Hyup | - |
dc.contributor.affiliatedAuthor | Choi, Sung Hoon | - |
dc.contributor.affiliatedAuthor | Shin, Hye Won | - |
dc.contributor.affiliatedAuthor | Kim, Do Young | - |
dc.contributor.affiliatedAuthor | Ro, Simon Weonsang | - |
dc.citation.volume | 16 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 280 | - |
dc.citation.endPage | 287 | - |
dc.identifier.bibliographicCitation | Korean Journal of Hepatology (대한간학회지), Vol.16(3) : 280-287, 2010 | - |
dc.identifier.rimsid | 54652 | - |
dc.type.rims | ART | - |
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