The surface profile and biocompatibility of hydroxyapatiteblasted gold membrane as a periodontal guided tissue regeneration membrane

Abstract

Guided tissue regeneration (GTR) membrane is essential for periodontal surgery, GTR membrane can guide successful bone tissue formation. The aim of this study is to assess morphologic property and biocompatibility of hydroxyapatite (HA) blasted gold membrane as a periodontal GTR membrane. 99.99 gold (LS Nikko, Korea) was rolled to 0.025∼0.050 mm thickness, and then, blasted with hydroxyapatite powder (5 ∼ 25 µm, Ossgen, Korea) onto the rolled gold sheets. Contact angle measurement of non-blasted and HA-blasted surface was performed. And, scanning electron microscopy analysis (simple surface profile observation and observation after attachment of human osteosarcoma cell) was performed. In implantation test using adult dog, Ti-membrane (Implantium, Suwon, Korea) was used as a control group, and HA-blasted gold membrane was used as a experimental group. Contact angle of HA-blasted gold membrane was higher than that of non-blasted gold surface (p < 0.05). In SEM analysis, HA-blasted gold membrane showed roughened surface and attachment of MG-63 cell was more than that of non-blasted gold surface. There were not any specific clinical symptoms and histological signs in implantation test. This result shows that HA-blasted gold membrane has a possibility as promising periodontal GTR membrane.