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DNA microarray-based identification of bacterial and fungal pathogens in bloodstream infections

Authors
 Seung Min Yoo  ;  Jun Yong Choi  ;  Jung Kuk Yun  ;  Jae Kyung Choi  ;  So Youn Shin  ;  Kyungwon Lee  ;  June Myung Kim  ;  Sang Yup Lee 
Citation
 MOLECULAR AND CELLULAR PROBES, Vol.24(1) : 44-52, 2010 
Journal Title
 MOLECULAR AND CELLULAR PROBES 
ISSN
 0890-8508 
Issue Date
2010
MeSH
Bacteria/classification* ; Bacteria/genetics ; Bacteria/isolation & purification ; DNA, Bacterial/blood* ; DNA, Bacterial/genetics ; DNA, Fungal/blood* ; DNA, Fungal/genetics ; Fungi/classification* ; Fungi/genetics ; Fungi/isolation & purification ; Humans ; Oligonucleotide Array Sequence Analysis*
Keywords
DNA microarray ; Bloodstream infection ; Microbial pathogen ; DNA probes
Abstract
The accurate and rapid identification of pathogens in blood is a major challenge in clinical pathogen diagnostics because of the high mortality of sepsis. Here we report the development of DNA microarray for the identification of pathogens causing bloodstream infections. Species-specific and bacteria- and fungi-broad-ranged probes were designed to identify 50 bacteria and 7 fungi. The specificities and sensitivities of the selected probes were successfully validated by applying reference strains. To assess the performance of the DNA microarray in a clinical setting, blind tests were performed using 112 blood culture specimens that showed preliminary presence of pathogenic microorganisms by culture-based method, resulting in the correct identification of pathogens in 104 samples showing the sensitivity of 93%. In addition, closely-related species could be discriminated by the distinct hybridization patterns. This DNA microarray-based pathogen diagnosis takes approximately 10 h starting from a positive blood culture, considerably reducing time required to sufficiently identify pathogens by subsequent agar-culture and biochemical tests which requires altogether at least 1-3 days. Also, the amount of sample required for the identification of pathogens is much less than that required for biochemical assays. Thus, the DNA microarray reported here should be useful for the effective identification of microbial pathogens in blood cultures from septicemic patients
Full Text
http://www.sciencedirect.com/science/article/pii/S0890850809000619
DOI
10.1016/j.mcp.2009.09.003
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kim, June Myung(김준명)
Shin, So Youn(신소연)
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
Choi, Jun Yong(최준용) ORCID logo https://orcid.org/0000-0002-2775-3315
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/101570
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