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Genetic diversity of chromosomal metallo-beta-lactamase genes in clinical isolates of Elizabethkingia meningoseptica from Korea

Authors
 Jong Hwa Yum  ;  Eun Young Lee  ;  Sung-Ho Hur  ;  Seok Hoon Jeong  ;  Hyukmin Lee  ;  Dongeun Yong  ;  Yunsop Chong  ;  Eun-Woo Lee  ;  Patrice Nordmann  ;  Kyungwon Lee 
Citation
 JOURNAL OF MICROBIOLOGY, Vol.48(3) : 358-364, 2010 
Journal Title
 JOURNAL OF MICROBIOLOGY 
ISSN
 1225-8873 
Issue Date
2010
MeSH
Adult ; Aged ; Aged, 80 and over ; Amino Acid Sequence ; Bacterial Proteins/classification ; Bacterial Proteins/genetics* ; Base Sequence ; Child ; Child, Preschool ; Chromosomes, Bacterial/enzymology ; Chromosomes, Bacterial/genetics ; Chryseobacterium/drug effects ; Chryseobacterium/enzymology* ; Chryseobacterium/genetics* ; DNA Primers/genetics ; DNA, Bacterial/genetics ; Drug Resistance, Bacterial ; Female ; Flavobacteriaceae Infections/microbiology ; Genes, Bacterial* ; Genetic Variation ; Genotype ; Humans ; Infant ; Infant, Newborn ; Korea ; Male ; Middle Aged ; Molecular Sequence Data ; Sequence Homology, Amino Acid ; Young Adult ; beta-Lactamases/classification ; beta-Lactamases/genetics*
Keywords
E. meningoseptica  ; metallo-β-lactamase ;  GOB ;  BlaB ;  cluster 
Abstract
This study was performed to characterize the chromosomal metallo-beta-lactamases (MBLs) of Elizabethkingia meningoseptica isolated from Korea and to propose a clustering method of BlaB and GOB MBLs based on their amino acid similarities. Chromosomal MBL genes were amplified by PCR from 31 clinical isolates of E. meningoseptica. These PCR products were then cloned into a vector and electrotransformed into E. coli DH5 alpha. Nucleotide sequencing was performed by the dideoxy chain termination method using PCR products or cloned DNA fragments. Antimicrobial susceptibilities were determined by the agar dilution method. PCR experiments showed that all 31 E. meningoseptica isolates contained both the blaB and the bla (GOB) genes. DNA sequence analysis revealed that E. meningoseptica isolates possessed seven types of blaB gene, including five novel variants (blaB-9 to blaB-13) and 11 types of bla (GOB) gene, including 10 novel variants (bla (GOB-8) to bla (GOB-17)). The most common combination of MBL was BlaB-12 plus GOB-17 (n=19). Minimum inhibitory concentrations of imipenem and meropenem for the electrotransformants harboring novel BlaB and GOB MBLs were two- or four-fold higher than those for the recipient E. coli DH5 alpha. BlaB and GOB MBLs were grouped in three and six clusters including fifteen novel variants, respectively, based on amino acid similarities
Full Text
http://link.springer.com/article/10.1007%2Fs12275-010-9308-5
DOI
10.1007/s12275-010-9308-5
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
Jeong, Seok Hoon(정석훈) ORCID logo https://orcid.org/0000-0001-9290-897X
Chong, Yun Sop(정윤섭)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/101502
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