1 239

Cited 15 times in

Genetic diversity of chromosomal metallo-beta-lactamase genes in clinical isolates of Elizabethkingia meningoseptica from Korea

DC FieldValueLanguage
dc.contributor.author정윤섭-
dc.contributor.author용동은-
dc.contributor.author이경원-
dc.contributor.author정석훈-
dc.date.accessioned2015-04-23T16:55:54Z-
dc.date.available2015-04-23T16:55:54Z-
dc.date.issued2010-
dc.identifier.issn1225-8873-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/101502-
dc.description.abstractThis study was performed to characterize the chromosomal metallo-beta-lactamases (MBLs) of Elizabethkingia meningoseptica isolated from Korea and to propose a clustering method of BlaB and GOB MBLs based on their amino acid similarities. Chromosomal MBL genes were amplified by PCR from 31 clinical isolates of E. meningoseptica. These PCR products were then cloned into a vector and electrotransformed into E. coli DH5 alpha. Nucleotide sequencing was performed by the dideoxy chain termination method using PCR products or cloned DNA fragments. Antimicrobial susceptibilities were determined by the agar dilution method. PCR experiments showed that all 31 E. meningoseptica isolates contained both the blaB and the bla (GOB) genes. DNA sequence analysis revealed that E. meningoseptica isolates possessed seven types of blaB gene, including five novel variants (blaB-9 to blaB-13) and 11 types of bla (GOB) gene, including 10 novel variants (bla (GOB-8) to bla (GOB-17)). The most common combination of MBL was BlaB-12 plus GOB-17 (n=19). Minimum inhibitory concentrations of imipenem and meropenem for the electrotransformants harboring novel BlaB and GOB MBLs were two- or four-fold higher than those for the recipient E. coli DH5 alpha. BlaB and GOB MBLs were grouped in three and six clusters including fifteen novel variants, respectively, based on amino acid similarities-
dc.description.statementOfResponsibilityopen-
dc.format.extent358~364-
dc.relation.isPartOfJOURNAL OF MICROBIOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdult-
dc.subject.MESHAged-
dc.subject.MESHAged, 80 and over-
dc.subject.MESHAmino Acid Sequence-
dc.subject.MESHBacterial Proteins/classification-
dc.subject.MESHBacterial Proteins/genetics*-
dc.subject.MESHBase Sequence-
dc.subject.MESHChild-
dc.subject.MESHChild, Preschool-
dc.subject.MESHChromosomes, Bacterial/enzymology-
dc.subject.MESHChromosomes, Bacterial/genetics-
dc.subject.MESHChryseobacterium/drug effects-
dc.subject.MESHChryseobacterium/enzymology*-
dc.subject.MESHChryseobacterium/genetics*-
dc.subject.MESHDNA Primers/genetics-
dc.subject.MESHDNA, Bacterial/genetics-
dc.subject.MESHDrug Resistance, Bacterial-
dc.subject.MESHFemale-
dc.subject.MESHFlavobacteriaceae Infections/microbiology-
dc.subject.MESHGenes, Bacterial*-
dc.subject.MESHGenetic Variation-
dc.subject.MESHGenotype-
dc.subject.MESHHumans-
dc.subject.MESHInfant-
dc.subject.MESHInfant, Newborn-
dc.subject.MESHKorea-
dc.subject.MESHMale-
dc.subject.MESHMiddle Aged-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHSequence Homology, Amino Acid-
dc.subject.MESHYoung Adult-
dc.subject.MESHbeta-Lactamases/classification-
dc.subject.MESHbeta-Lactamases/genetics*-
dc.titleGenetic diversity of chromosomal metallo-beta-lactamase genes in clinical isolates of Elizabethkingia meningoseptica from Korea-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Laboratory Medicine (진단검사의학)-
dc.contributor.googleauthorJong Hwa Yum-
dc.contributor.googleauthorEun Young Lee-
dc.contributor.googleauthorSung-Ho Hur-
dc.contributor.googleauthorSeok Hoon Jeong-
dc.contributor.googleauthorHyukmin Lee-
dc.contributor.googleauthorDongeun Yong-
dc.contributor.googleauthorYunsop Chong-
dc.contributor.googleauthorEun-Woo Lee-
dc.contributor.googleauthorPatrice Nordmann-
dc.contributor.googleauthorKyungwon Lee-
dc.identifier.doi10.1007/s12275-010-9308-5-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA03679-
dc.contributor.localIdA02423-
dc.contributor.localIdA02649-
dc.contributor.localIdA03619-
dc.relation.journalcodeJ01593-
dc.identifier.eissn1976-3794-
dc.identifier.pmid20571954-
dc.identifier.urlhttp://link.springer.com/article/10.1007%2Fs12275-010-9308-5-
dc.subject.keywordE. meningoseptica -
dc.subject.keywordmetallo-β-lactamase-
dc.subject.keyword GOB-
dc.subject.keyword BlaB-
dc.subject.keyword cluster -
dc.contributor.alternativeNameChong, Yun Sop-
dc.contributor.alternativeNameYong, Dong Eun-
dc.contributor.alternativeNameLee, Kyung Won-
dc.contributor.alternativeNameJeong, Seok Hoon-
dc.contributor.affiliatedAuthorChong, Yun Sop-
dc.contributor.affiliatedAuthorYong, Dong Eun-
dc.contributor.affiliatedAuthorLee, Kyung Won-
dc.contributor.affiliatedAuthorJeong, Seok Hoon-
dc.citation.volume48-
dc.citation.number3-
dc.citation.startPage358-
dc.citation.endPage364-
dc.identifier.bibliographicCitationJOURNAL OF MICROBIOLOGY, Vol.48(3) : 358-364, 2010-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.