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새로운 색지표를 이용한 우식활성검사법 개발

Other Titles
 Development of a new color scale for a caries activity test 
Authors
 강시묵  ;  정회인  ;  정승화  ;  권호근  ;  김백일 
Citation
 Journal of Korean Academy of Oral Health (대한구강보건학회지), Vol.34(1) : 9-17, 2010 
Journal Title
Journal of Korean Academy of Oral Health(대한구강보건학회지)
ISSN
 1225-388X 
Issue Date
2010
Keywords
caries ; caries activity test ; cariogenic bacteria ; cariostat ; pH indicator
Abstract
Objectives. The aim of this study was to develop a new caries activity test based on colorimetric changes that could better reflect
the amount of acid produced by oral bacteria as a broad spectrum of color than the previous test. The optimal candidates were
combinations of pH indicators, and these were evaluated in pH buffering solutions and using dental plaque.
Methods. Six pH indicators, Bromocresol-purple (BCP), Bromocresol-green (BCG), Methyl-red (MR), Methyl-orange (MO),
Resazurin (R), and Naphthyl-red (NR), were selected to show different colors at various pH environments (range pH 3.0∼7.0).
BCP and BCG are pH indicators that are already used in the CariostatⓇ, and they can show color changes from blue to yellow.
This study tried to broaden the color change spectrum from blue to red to give a more distinct differentiation at various pHs.
Four pH indicators (MR, MO, R and NR) were blended with BCP and BCG. Each combination of the three indicators was assessed
in different pH buffer systems: pH 7.0, 6.0, 5.0, 4.0, and 3.0. The selected combinations of pH indicators were applied to human
dental plaque from 11 subjects (mean age: 27) to confirm the reproducibility of the in vitro results.
Results. According to the in vitro buffer system, the mixture of BCP, BCG and MR did not show any differences between pH
4.0 and pH 3.0. On the other hand, the mixtures of BCP-BCG-MO (ratio 2:1:1.5), BCP-BCG-R (1.5:1:0.5) and BCP-BCG-NR
(2:1:1.5) showed distinguishable color changes from pH 7.0 to pH 3.0. Among the three candidates, the BCP-BCG-NR mixture
showed the best color differences in the buffer solution and the human dental plaque cultivated solutions at various pHs.
Conclusions. This study evaluated a new colorimetric caries activity test that used a combination of several pH indicators. The
new system can easily detect various pH environments from organic acid fermentation by using a wider range of colors
Files in This Item:
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Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Preventive Dentistry and Public Oral Health (예방치과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Others (기타) > 1. Journal Papers
Yonsei Authors
Kang, Si-Mook(강시묵) ORCID logo https://orcid.org/0000-0003-2341-3636
Kwon, Ho Keun(권호근)
Kim, Baek Il(김백일) ORCID logo https://orcid.org/0000-0001-8234-2327
Jeong, Seung Hwa(정승화)
Jung, Hoi In(정회인) ORCID logo https://orcid.org/0000-0002-1978-6926
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/101428
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