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Remifentanil protects myocardium through activation of anti-apoptotic pathways of survival in ischemia-reperfused rat heart

 H. S. KIM  ;  J. E. CHO  ;  S. W. HONG  ;  S. O. KIM  ;  J. K. SHIM  ;  Y. L. KWAK 
 PHYSIOLOGICAL RESEARCH, Vol.59(3) : 347-356, 2010 
Journal Title
Issue Date
Adjuvants, Anesthesia/administration & dosage ; Adjuvants, Anesthesia/pharmacology* ; Animals ; Apoptosis/drug effects* ; Blotting, Western ; Calcium/metabolism ; Cell Survival/drug effects ; Cytochromes c/metabolism ; Disease Models, Animal ; Gene Expression Regulation/drug effects ; Hemodynamics/drug effects ; Homeostasis ; Male ; Mitogen-Activated Protein Kinase 1/metabolism ; Mitogen-Activated Protein Kinase 3/metabolism ; Myocardial Infarction/genetics ; Myocardial Infarction/metabolism ; Myocardial Infarction/pathology ; Myocardial Infarction/physiopathology ; Myocardial Infarction/prevention & control* ; Myocardial Reperfusion Injury/genetics ; Myocardial Reperfusion Injury/metabolism ; Myocardial Reperfusion Injury/pathology ; Myocardial Reperfusion Injury/physiopathology ; Myocardial Reperfusion Injury/prevention & control* ; Myocardium/metabolism ; Myocardium/pathology* ; Phosphorylation ; Piperidines/administration & dosage ; Piperidines/pharmacology* ; Proto-Oncogene Proteins c-bcl-2/metabolism ; RNA, Messenger/metabolism ; Rats ; Rats, Sprague-Dawley ; Reverse Transcriptase Polymerase Chain Reaction ; Sarcoplasmic Reticulum/metabolism ; Time Factors ; bcl-2-Associated X Protein/metabolism
Anti-apoptosis ; Cardioprotection ; Ischemia/ reperfusion injury ; Remifentanil
Remifentanil is a commonly used opioid in anesthesia with cardioprotective effect in ischemia-reperfused (I/R) heart. We evaluated the influence of remifentanil on myocardial infarct size and expressions of proteins involved in apoptosis in I/R rat heart following various time protocols of remifentanil administration. Artificially ventilated anesthetized Sprague-Dawley rats were subjected to a 30 min of left anterior descending coronary artery occlusion followed by 2 h of reperfusion. Rats were randomly assigned to one of five groups; Sham, I/R only, remifentanil preconditioning, postconditioning and continuous infusion group. Myocardial infarct size, the phosphorylation of ERK1/2, Bcl2, Bax and cytochrome c and the expression of genes influencing Ca2+ homeostasis were assessed. In remifentanil-administered rat hearts, regardless of the timing and duration of administration, infarct size was consistently reduced compared to I/R only rats. Remifentanil improved expression of ERK1/2 and anti-apoptotic protein Bcl2, and expression of sarcoplasmic reticulum genes which were significantly reduced in the I/R rats only. Remifentanil reduced expression of pro-apoptotic protein, Bax and cytochrome c. These suggested that remifentanil produced cardioprotective effect by preserving the expression of proteins involved in anti-apoptotic pathways, and the expression of sarcoplasmic reticulum genes in I/R rat heart, regardless of the timing of administration.
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1. College of Medicine (의과대학) > Dept. of Anesthesiology and Pain Medicine (마취통증의학교실) > 1. Journal Papers
Yonsei Authors
Kwak, Young Lan(곽영란) ORCID logo https://orcid.org/0000-0002-2984-9927
Shim, Jae Kwang(심재광) ORCID logo https://orcid.org/0000-0001-9093-9692
Cho, Jang Eun(조장은)
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