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Induction of Vascular Endothelial Growth Factor Protein by Mycoplasma pneumoniae

Other Titles
 호흡기상피세포 내 Mycoplasma pneumoniae에 의한 혈관내피세포성장인자의 유도 
Authors
 이경은  ;  홍정연  ;  손명현  ;  김경원  ;  박인달  ;  장명웅  ;  김규언 
Citation
 Pediatric Allergy and Respiratory Disease (소아알레르기 호흡기학회지), Vol.20(2) : 100-106, 2010 
Journal Title
 Pediatric Allergy and Respiratory Disease (소아알레르기 호흡기학회지) 
ISSN
 1225-679x 
Issue Date
2010
Keywords
Mycoplasma pneumoniae ; Vascular endothelial growth factor ; Mitogen-activated protein kinase ; Airway epithelial cell
Abstract
Background:Mycoplasma pneumoniae is an extracellular pathogen that attaches to and destroys the ciliated epithelial cells of the respiratory tract. The vascular endothelial growth factor (VEGF) is a critical angiogenic factor that manages the formation and function of vascular networks. Thus, we examined whether M. pneumoniae lysate (MPL) induces VEGF and MPL-induced VEGF expression is regulated by the activation of mitogen-activated protein kinase (MAPK) pathways in airway epithelial cells. Methods:Cells were treated with MPL in dose and time dependent manners or pretreated with chemical inhibitors of MAPK signaling molecules before the addition of MPL. The supernatants were measured by a specific human VEGF enzyme-linked immunosorbent assay (ELISA). The RNAs were extracted and synthesized into cDNAs for VEGF gene expression by polymerase chain reaction. Results:MPL considerably increased VEGF mRNA 2 hours after treatment, which was gradually reduced thereafter. On the other hand, VEGF protein was continuously amplified for 12 hours after both 5 and 10 μg/mL MPL treatment. Pretreatment with U0126 (a specific extracellular signal-regulated kinase inhibitor) and SB202190 (a specific p38 inhibitor) abolished MPL-stimulated VEGF protein close to basal level (-85%), whereas JNK inhibitor II (a specific c-Jun N-terminal kinase inhibitor) partially decreased VEGF protein (57%). Conclusions:We concluded that MPL induces VEGF expression through the activation of MAPK signaling molecules (ERK, p38 and JNK) in airway epithelial cells
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Won(김경원) ORCID logo https://orcid.org/0000-0003-4529-6135
Kim, Kyu Earn(김규언)
Sohn, Myung Hyun(손명현) ORCID logo https://orcid.org/0000-0002-2478-487X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/101396
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