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Induction of Vascular Endothelial Growth Factor Protein by Mycoplasma pneumoniae
DC Field | Value | Language |
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dc.contributor.author | 김경원 | - |
dc.contributor.author | 김규언 | - |
dc.contributor.author | 손명현 | - |
dc.date.accessioned | 2015-04-23T16:52:37Z | - |
dc.date.available | 2015-04-23T16:52:37Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 1225-679x | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/101396 | - |
dc.description.abstract | Background:Mycoplasma pneumoniae is an extracellular pathogen that attaches to and destroys the ciliated epithelial cells of the respiratory tract. The vascular endothelial growth factor (VEGF) is a critical angiogenic factor that manages the formation and function of vascular networks. Thus, we examined whether M. pneumoniae lysate (MPL) induces VEGF and MPL-induced VEGF expression is regulated by the activation of mitogen-activated protein kinase (MAPK) pathways in airway epithelial cells. Methods:Cells were treated with MPL in dose and time dependent manners or pretreated with chemical inhibitors of MAPK signaling molecules before the addition of MPL. The supernatants were measured by a specific human VEGF enzyme-linked immunosorbent assay (ELISA). The RNAs were extracted and synthesized into cDNAs for VEGF gene expression by polymerase chain reaction. Results:MPL considerably increased VEGF mRNA 2 hours after treatment, which was gradually reduced thereafter. On the other hand, VEGF protein was continuously amplified for 12 hours after both 5 and 10 μg/mL MPL treatment. Pretreatment with U0126 (a specific extracellular signal-regulated kinase inhibitor) and SB202190 (a specific p38 inhibitor) abolished MPL-stimulated VEGF protein close to basal level (-85%), whereas JNK inhibitor II (a specific c-Jun N-terminal kinase inhibitor) partially decreased VEGF protein (57%). Conclusions:We concluded that MPL induces VEGF expression through the activation of MAPK signaling molecules (ERK, p38 and JNK) in airway epithelial cells | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 100~106 | - |
dc.relation.isPartOf | Pediatric Allergy and Respiratory Disease (소아알레르기 호흡기학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Induction of Vascular Endothelial Growth Factor Protein by Mycoplasma pneumoniae | - |
dc.title.alternative | 호흡기상피세포 내 Mycoplasma pneumoniae에 의한 혈관내피세포성장인자의 유도 | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pediatrics (소아과학) | - |
dc.contributor.googleauthor | 이경은 | - |
dc.contributor.googleauthor | 홍정연 | - |
dc.contributor.googleauthor | 손명현 | - |
dc.contributor.googleauthor | 김경원 | - |
dc.contributor.googleauthor | 박인달 | - |
dc.contributor.googleauthor | 장명웅 | - |
dc.contributor.googleauthor | 김규언 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00303 | - |
dc.contributor.localId | A00327 | - |
dc.contributor.localId | A01967 | - |
dc.relation.journalcode | J02476 | - |
dc.subject.keyword | Mycoplasma pneumoniae | - |
dc.subject.keyword | Vascular endothelial growth factor | - |
dc.subject.keyword | Mitogen-activated protein kinase | - |
dc.subject.keyword | Airway epithelial cell | - |
dc.contributor.alternativeName | Kim, Kyung Won | - |
dc.contributor.alternativeName | Kim, Kyu Earn | - |
dc.contributor.alternativeName | Son, Myung Hyun | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Won | - |
dc.contributor.affiliatedAuthor | Kim, Kyu Earn | - |
dc.contributor.affiliatedAuthor | Son, Myung Hyun | - |
dc.citation.volume | 20 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 100 | - |
dc.citation.endPage | 106 | - |
dc.identifier.bibliographicCitation | Pediatric Allergy and Respiratory Disease (소아알레르기 호흡기학회지), Vol.20(2) : 100-106, 2010 | - |
dc.identifier.rimsid | 51026 | - |
dc.type.rims | ART | - |
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