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Functional evaluation of chondrocyte sheeting immunodelusive immunoisolated bioartificial pancreas

 J.Y. Kim  ;  J.-I. Lee  ;  J.H. Jeong  ;  Y.-J. Park  ;  S.J. Kim  ;  Y.H. Fang  ;  Y. Cho  ;  K.H. Huh  ;  D.J. Joo  ;  M.S. Kim  ;  Y.S. Kim 
 TRANSPLANTATION PROCEEDINGS, Vol.42(3) : 903-906, 2010 
Journal Title
Issue Date
Animals ; Bioartificial Organs* ; Cartilage/physiology ; Cell Culture Techniques ; Chondrocytes/cytology* ; Chondrocytes/physiology ; Glucose/pharmacology ; Insulin/secretion ; Islets of Langerhans/cytology ; Islets of Langerhans/physiology ; Islets of Langerhans/secretion* ; Pancreas/physiology* ; Rats ; Rats, Inbred Lew ; Rats, Sprague-Dawley
In islet transplantation, encapsulation of immunoisolated islets may provide a way to protect the graft from immune attacks with no immunosuppression. To develop an immunodelusive immunoisolated bioartificial pancreas (BAP), chondrocyte sheets were prepared by cell sheet engineering. We made an immunoisolated BAP encapsulated with rodent-derived chondrocyte sheets and then evaluated its function. Sprague-Dawley rats were used as the source of auricular cartilage and chondrocytes were maintained and expanded by passages. Lewis rats were prepared for islet isolation. A 3-dimensional chondrocyte sheeting immunodelusive immunoisolated BAP (CSI-BAP) was created by multi-layering and unifying the chondrocyte sheets. Subsequently, islets were embedded between each multi-layer sheet. To evaluate the function of the CSI-BAP, a glucose challenge test was performed and secretion of insulin in the culture medium was measured by an enzyme-linked immunosorbent assay. When observed by phase-contrast microscopy, the CSI-BAP maintained close connections between chondrocyte sheets. Islets in the CSI-BAP maintained viability at day 10 and showed good insulin secretion, as revealed by a prompt reaction to increased concentrations of glucose at days 5 and 10. In long-term culture, the CSI-BAP maintained its ability to secrete insulin for 8 weeks. This BAP technology could be an important tool for successful islet transplantation without immunosuppressive drugs.
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1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kim, Myoung Soo(김명수) ORCID logo https://orcid.org/0000-0002-8975-8381
Kim, Yu Seun(김유선) ORCID logo https://orcid.org/0000-0002-5105-1567
Kim, Joon Ye(김준예)
Park, Yun Jong(박윤종)
Fang, Yu Hui(방우휘)
Jeong, Jin Ho(정진호)
Joo, Dong Jin(주동진) ORCID logo https://orcid.org/0000-0001-8405-1531
Huh, Kyu Ha(허규하) ORCID logo https://orcid.org/0000-0003-1364-6989
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