3 331

Cited 52 times in

Hypoxia-preconditioned adipose tissue-derived mesenchymal stem cell increase the survival and gene expression of engineered neural stem cells in a spinal cord injury model

 Jin Soo Oh  ;  Yoon Ha  ;  Sung Su An  ;  Momin Khan  ;  William A. Pennant  ;  Hyo Jin Kim  ;  Do Heum Yoon  ;  Minhyung Lee  ;  Keung Nyun Kim 
 NEUROSCIENCE LETTERS, Vol.472(3) : 215-219, 2010 
Journal Title
Issue Date
Adipose Tissue/cytology* ; Animals ; Cell Hypoxia ; Cell Survival ; Gene Expression ; Genetic Engineering ; Mesenchymal Stem Cell Transplantation ; Nerve Tissue/cytology* ; Rats ; Spinal Cord Injuries/metabolism* ; Spinal Cord Injuries/pathology* ; Spinal Cord Injuries/therapy ; Stem Cell Transplantation*
Hypoxia preconditioning ; Stem cell therapy ; Gene therapy ; Co-transplantation ; Spinal cord injury
Hypoxic preconditioning (HP) is a novel strategy to make stem cells resistant to the ischemic environment they encounter after transplantation into injured tissue; this strategy improves survival of both the transplanted cells and the host cells at the injury site. Using both in vitro and in vivo injury models, we confirmed that HP-treated adipose tissue-derived mesenchymal stem cells (HP-AT-MSCs) increased cell survival and enhanced the expression of marker genes in DsRed-engineered neural stem cells (NSCs-DsRed). Similar to untreated AT-MSCs, HP-AT-MSCs had normal morphology and were positive for the cell surface markers CD90, CD105, and CD29, but not CD31. In three in vitro ischemic-mimicking injury models, HP-AT-MSCs significantly increased both the viability of NSCs-DsRed and the expression of DsRed and clearly reduced the number of annexin-V-positive apoptotic NSCs-DsRed and the expression of the apoptotic factor Bax. Consistent with the in vitro assay, co-transplantation of NSCs-DsRed with HP-AT-MSCs significantly improved the survival of the NSCs-DsRed, resulting in an increased expression of the DsRed reporter gene at the transplantation site in a rat spinal cord injury (SCI) model. These findings suggest that the co-transplantation of HP-AT-MSCs with engineered NSCs can improve both the cell survival and the gene expression of the engineered NSCs, indicating that this novel strategy can be used to augment the therapeutic efficacy of combined stem cell and gene therapies for SCI.
Full Text
Appears in Collections:
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Keung Nyun(김긍년)
Kim, Hyo Jin(김효진)
An, Sung Su(안성수)
Oh, Jin Soo(오진수)
Yoon, Do Heum(윤도흠) ORCID logo https://orcid.org/0000-0003-1452-5724
Ha, Yoon(하윤)
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.