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An 8-gene signature, including methylated and down-regulated glutathione peroxidase 3, of gastric cancer

 Xianglan Zhang  ;  Jung-Jin Yang  ;  Yoon Sun Kim  ;  Ki-Yeol Kim  ;  Woong Shick Ahn  ;  Sanghwa Yang 
 International Journal of Oncology, Vol.36(2) : 405-414, 2010 
Journal Title
 International Journal of Oncology 
Issue Date
Adenocarcinoma/genetics* ; Adenocarcinoma/metabolism ; Adult ; Aged ; Biomarkers, Tumor/genetics* ; Chromogranin A/biosynthesis ; Chromogranin A/genetics ; DNA Methylation/genetics* ; Down-Regulation ; Gene Expression Profiling ; Glutathione Peroxidase/biosynthesis ; Glutathione Peroxidase/genetics* ; HSP47 Heat-Shock Proteins/biosynthesis ; HSP47 Heat-Shock Proteins/genetics ; Humans ; Immunohistochemistry ; Inhibin-beta Subunits/biosynthesis ; Inhibin-beta Subunits/genetics ; Kaplan-Meier Estimate ; Kruppel-Like Transcription Factors/biosynthesis ; Kruppel-Like Transcription Factors/genetics ; Lipase/biosynthesis ; Lipase/genetics ; Middle Aged ; Neoplasm Staging ; Oligonucleotide Array Sequence Analysis ; Promoter Regions, Genetic ; Reverse Transcriptase Polymerase Chain Reaction ; Somatostatin/biosynthesis ; Somatostatin/genetics ; Stomach Neoplasms/genetics* ; Stomach Neoplasms/metabolism ; Thy-1 Antigens/biosynthesis ; Thy-1 Antigens/genetics ; Tissue Array Analysis ; Up-Regulation
gastric cancer ; biomarker ; gene expression profiling ; methylation
We have identified an 8-gene signature with significant expression differences between gastric cancer and normal gastric tissues. This 8-gene set can predict the normal and cancer status of gastric tissues with more than 96% accuracy in a totally independent microarray dataset. The 8 genes are composed of down-regulated KLF4, GPX3, SST and LIPF, together with up-regulated SERPINH1, THY1 and INHBA in gastric cancer. To corroborate the differential gene expression pattern, we chose GPX3 and examined its expression pattern in detail. A comparison of GPX3 expression pattern shows a broader down-regulated pattern in multiple types of cancers, including cervical, thyroid, head and neck, lung cancers and melanoma than in healthy controls. An immuno-histostaining analysis in tissue microarrays confirms GPX3 down-regulation in gastric cancer. Mechanism-wise GPX3 down-regulation in gastric cancer is due to promoter hypermethylation. Collectively, these results show a correct identification of 8 genes as gastric cancer biomarkers.
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5. Research Institutes (연구소) > Oral Cancer Research Institute (구강종양연구소) > 1. Journal Papers
Yonsei Authors
Kim, Ki Yeol(김기열) ORCID logo https://orcid.org/0000-0001-5357-1067
Zhang, Xiang Lan(장향란)
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