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Cordyceps pruinosa extracts induce apoptosis of HeLa cells by a caspase dependent pathway

Authors
 Ho Gyoung Kim  ;  Heesang Song  ;  Deok Hyo Yoon  ;  Byeong-Wook Song  ;  Sang Min Park  ;  Gi Ho Sung  ;  Jae-Youl Cho  ;  Hae Il Park  ;  Sunga Choi  ;  Won O Song  ;  Ki-Chul Hwang  ;  Tae Woong Kim 
Citation
 JOURNAL OF ETHNOPHARMACOLOGY, Vol.128(2) : 342-351, 2010 
Journal Title
 JOURNAL OF ETHNOPHARMACOLOGY 
ISSN
 0378-8741 
Issue Date
2010
MeSH
Apoptosis/drug effects* ; Caspase 3/metabolism* ; Caspases/metabolism* ; Cordyceps/metabolism* ; Cytochrome c Group/metabolism ; Cytochromes c/metabolism ; DNA Fragmentation/drug effects ; HeLa Cells ; Humans ; Mitochondria/metabolism ; Plant Extracts/pharmacology* ; Poly(ADP-ribose) Polymerases/metabolism ; Reactive Oxygen Species/metabolism ; Signal Transduction/drug effects ; bcl-2-Associated X Protein/metabolism
Keywords
Cordyceps pruinosa ; Anti-proliferation ; Apoptosis ; HeLa cells
Abstract
AIM OF THE STUDY: Cordyceps is a parasitic fungus and has long been used as a traditional Chinese medicine to treat illnesses, promote longevity, increase athletic power, and relieve exhaustion and cancer. In this study, we reveal the mechanisms underlying apoptosis induced by Cordyceps pruinosa butanol fraction (CPBF) in the human cervical adenocarcinoma cell line, HeLa. MATERIALS AND METHODS: Proliferation and apoptosis of cells were examined by MTT assay, DNA fragmentation, phosphatidyl serine distribution assay, Western blot analysis, and immunocytochemistry. To determine the association between CPBF related apoptosis and ROS, electron spin resonance (ESR) trapping experiments were used. RESULTS: CPBF inhibited proliferation and induced apoptosis in HeLa cells in a dose-dependent manner using a MTT assay, DNA fragmentation, and a phosphatidyl serine distribution assay. Western blot analysis showed that apoptosis in HeLa cells was caspase-3- and -9-dependent. Proteolytic cleavage of PARP and the release of cytochrome c from the mitochondria into the cytosol were significantly increased and the Bcl-2/Bax protein ratio was decreased. Apoptosis induced by CPBF was not prevented by various antioxidants. CONCLUSIONS: These results indicate that apoptotic effects of CPBF on HeLa cells are mediated by mitochondria-dependent death-signaling pathway independent of reactive oxygen species, suggesting that CPBF might be effective as an anti-proliferative agent for cancer
Full Text
http://www.sciencedirect.com/science/article/pii/S0378874110000802
DOI
10.1016/j.jep.2010.01.049
Appears in Collections:
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Song, Byeong Wook(송병욱)
Hwang, Ki Chul(황기철)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/100823
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