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Cell-penetrating DNA-binding protein as a safe and efficient naked DNA delivery carrier in vitro and in vivo

Authors
 Eun-Sung Kim  ;  Seung-Woo Yang  ;  Dong-Ki Hong  ;  Woo-Taek Kim  ;  Ho-Guen Kim  ;  Sang-Kyou Lee 
Citation
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.392(1) : 9-15, 2010 
Journal Title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN
 0006-291X 
Issue Date
2010
MeSH
Carrier Proteins/genetics ; Carrier Proteins/metabolism* ; DNA/genetics ; DNA/metabolism* ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism* ; HeLa Cells ; Humans ; Polycomb Repressive Complex 1 ; Recombinant Fusion Proteins/genetics ; Recombinant Fusion Proteins/metabolism* ; Saccharomyces cerevisiae Proteins/genetics ; Saccharomyces cerevisiae Proteins/metabolism* ; Transcription Factors/genetics ; Transcription Factors/metabolism* ; Transduction, Genetic/methods*
Keywords
DNA delivery ; Protein transduction domain ; DNA-binding domain ; Upstream activating sequence ; Intranasal delivery
Abstract
Non-viral gene delivery is a safe and suitable alternative to viral vector-mediated delivery to overcome the immunogenicity and tumorigenesis associated with viral vectors. Using the novel, human-origin Hph-1 protein transduction domain that can facilitate the transduction of protein into cells, we developed a new strategy to deliver naked DNA in vitro and in vivo. The new DNA delivery system contains Hph-1-GAL4 DNA-binding domain (DBD) fusion protein and enhanced green fluorescent protein (EGFP) reporter plasmid that includes the five repeats of GAL4 upstream activating sequence (UAS). Hph-1-GAL4-DBD protein formed complex with plasmid DNA through the specific interaction between GAL4-DBD and UAS, and delivered into the cells via the Hph-1-PTD. The pEGFP DNA was successfully delivered by the Hph-1-GAL4 system, and the EGFP was effectively expressed in mammalian cells such as HeLa and Jurkat, as well as in Bright Yellow-2 (BY-2) plant cells. When 10 microg of pEGFP DNA was intranasally administered to mice using Hph-1-GAL4 protein, a high level of EGFP expression was detected throughout the lung tissue for 7 days. These results suggest that an Hph-1-PTD-mediated DNA delivery strategy may be an useful non-viral DNA delivery system for gene therapy and DNA vaccines.
Full Text
http://www.sciencedirect.com/science/article/pii/S0006291X09025224
DOI
10.1016/j.bbrc.2009.12.135
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hogeun(김호근)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/100666
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