Cited 17 times in
Cell-penetrating DNA-binding protein as a safe and efficient naked DNA delivery carrier in vitro and in vivo
DC Field | Value | Language |
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dc.contributor.author | 김호근 | - |
dc.date.accessioned | 2015-04-23T16:29:03Z | - |
dc.date.available | 2015-04-23T16:29:03Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/100666 | - |
dc.description.abstract | Non-viral gene delivery is a safe and suitable alternative to viral vector-mediated delivery to overcome the immunogenicity and tumorigenesis associated with viral vectors. Using the novel, human-origin Hph-1 protein transduction domain that can facilitate the transduction of protein into cells, we developed a new strategy to deliver naked DNA in vitro and in vivo. The new DNA delivery system contains Hph-1-GAL4 DNA-binding domain (DBD) fusion protein and enhanced green fluorescent protein (EGFP) reporter plasmid that includes the five repeats of GAL4 upstream activating sequence (UAS). Hph-1-GAL4-DBD protein formed complex with plasmid DNA through the specific interaction between GAL4-DBD and UAS, and delivered into the cells via the Hph-1-PTD. The pEGFP DNA was successfully delivered by the Hph-1-GAL4 system, and the EGFP was effectively expressed in mammalian cells such as HeLa and Jurkat, as well as in Bright Yellow-2 (BY-2) plant cells. When 10 microg of pEGFP DNA was intranasally administered to mice using Hph-1-GAL4 protein, a high level of EGFP expression was detected throughout the lung tissue for 7 days. These results suggest that an Hph-1-PTD-mediated DNA delivery strategy may be an useful non-viral DNA delivery system for gene therapy and DNA vaccines. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 9~15 | - |
dc.relation.isPartOf | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Carrier Proteins/genetics | - |
dc.subject.MESH | Carrier Proteins/metabolism* | - |
dc.subject.MESH | DNA/genetics | - |
dc.subject.MESH | DNA/metabolism* | - |
dc.subject.MESH | DNA-Binding Proteins/genetics | - |
dc.subject.MESH | DNA-Binding Proteins/metabolism* | - |
dc.subject.MESH | HeLa Cells | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Polycomb Repressive Complex 1 | - |
dc.subject.MESH | Recombinant Fusion Proteins/genetics | - |
dc.subject.MESH | Recombinant Fusion Proteins/metabolism* | - |
dc.subject.MESH | Saccharomyces cerevisiae Proteins/genetics | - |
dc.subject.MESH | Saccharomyces cerevisiae Proteins/metabolism* | - |
dc.subject.MESH | Transcription Factors/genetics | - |
dc.subject.MESH | Transcription Factors/metabolism* | - |
dc.subject.MESH | Transduction, Genetic/methods* | - |
dc.title | Cell-penetrating DNA-binding protein as a safe and efficient naked DNA delivery carrier in vitro and in vivo | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pathology (병리학) | - |
dc.contributor.googleauthor | Eun-Sung Kim | - |
dc.contributor.googleauthor | Seung-Woo Yang | - |
dc.contributor.googleauthor | Dong-Ki Hong | - |
dc.contributor.googleauthor | Woo-Taek Kim | - |
dc.contributor.googleauthor | Ho-Guen Kim | - |
dc.contributor.googleauthor | Sang-Kyou Lee | - |
dc.identifier.doi | 10.1016/j.bbrc.2009.12.135 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01183 | - |
dc.relation.journalcode | J00281 | - |
dc.identifier.eissn | 1090-2104 | - |
dc.identifier.pmid | 20043881 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0006291X09025224 | - |
dc.subject.keyword | DNA delivery | - |
dc.subject.keyword | Protein transduction domain | - |
dc.subject.keyword | DNA-binding domain | - |
dc.subject.keyword | Upstream activating sequence | - |
dc.subject.keyword | Intranasal delivery | - |
dc.contributor.alternativeName | Kim, Ho Keun | - |
dc.contributor.affiliatedAuthor | Kim, Ho Keun | - |
dc.citation.volume | 392 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 9 | - |
dc.citation.endPage | 15 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.392(1) : 9-15, 2010 | - |
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