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Profiling of phospholipids in lipoproteins by multiplexed hollow fiber flow field-flow fractionation and nanoflow liquid chromatography-tandem mass spectrometry

Authors
 Ju Yong Lee  ;  Hye Kyeong Min  ;  Donghoon Choi  ;  Myeong Hee Moon 
Citation
 JOURNAL OF CHROMATOGRAPHY A, Vol.1217(10) : 1660-1666, 2010 
Journal Title
 JOURNAL OF CHROMATOGRAPHY A 
ISSN
 0021-9673 
Issue Date
2010
MeSH
Chromatography, Liquid/methods* ; Fractionation, Field Flow/methods* ; Humans ; Lipoproteins/blood* ; Lipoproteins/isolation & purification ; Phospholipids/blood* ; Phospholipids/isolation & purification ; Tandem Mass Spectrometry/methods*
Keywords
Hollow fiber flow field-flow fractionation ; Multiplexed HF5 ; Lipoprotein separation ; Phospholipids ; nLC–ESI-MS–MS ; HDL ; LDL
Abstract
This study describes a coupled analytical method to carry out the systematic profiling of phospholipids (PLs) in high-density lipoproteins (HDL) and low-density lipoproteins (LDL) from human blood plasma. HDL and LDL of healthy human plasma samples were separated by size and collected on a semi-preparative scale using multiplexed hollow fiber flow field-flow fractionation (MxHF5). Phospholipid mixtures contained in the resulting HDL and LDL fractions were analyzed by shotgun nanoflow liquid chromatography-tandem mass spectrometry (nLC-ESI-MS-MS). We utilized a dual scan method for the separation and simultaneous characterization of complicated PL mixtures by nLC-ESI-MS-MS, such that phosphatidylcholine (PC) and phosphatidylethanolamine (PE) molecules were detected in positive ion mode in a first LC run. In a second LC run, phosphatidylinositol (PI), phosphatidylglycerol (PG), and phosphatidic acid (PA) were detected in negative ion mode. In this study, a total of 56 PLs from HDL and 52 PLs from LDL particles were characterized by their molecular structures from data dependent collision-induced dissociation (CID) experiments, and their relative abundances were compared.
Full Text
http://www.sciencedirect.com/science/article/pii/S0021967310000336
DOI
10.1016/j.chroma.2010.01.006
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Choi, Dong Hoon(최동훈) ORCID logo https://orcid.org/0000-0002-2009-9760
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/100628
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