Cited 36 times in
Profiling of phospholipids in lipoproteins by multiplexed hollow fiber flow field-flow fractionation and nanoflow liquid chromatography-tandem mass spectrometry
DC Field | Value | Language |
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dc.contributor.author | 최동훈 | - |
dc.date.accessioned | 2015-04-23T16:27:48Z | - |
dc.date.available | 2015-04-23T16:27:48Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0021-9673 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/100628 | - |
dc.description.abstract | This study describes a coupled analytical method to carry out the systematic profiling of phospholipids (PLs) in high-density lipoproteins (HDL) and low-density lipoproteins (LDL) from human blood plasma. HDL and LDL of healthy human plasma samples were separated by size and collected on a semi-preparative scale using multiplexed hollow fiber flow field-flow fractionation (MxHF5). Phospholipid mixtures contained in the resulting HDL and LDL fractions were analyzed by shotgun nanoflow liquid chromatography-tandem mass spectrometry (nLC-ESI-MS-MS). We utilized a dual scan method for the separation and simultaneous characterization of complicated PL mixtures by nLC-ESI-MS-MS, such that phosphatidylcholine (PC) and phosphatidylethanolamine (PE) molecules were detected in positive ion mode in a first LC run. In a second LC run, phosphatidylinositol (PI), phosphatidylglycerol (PG), and phosphatidic acid (PA) were detected in negative ion mode. In this study, a total of 56 PLs from HDL and 52 PLs from LDL particles were characterized by their molecular structures from data dependent collision-induced dissociation (CID) experiments, and their relative abundances were compared. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1660~1666 | - |
dc.relation.isPartOf | JOURNAL OF CHROMATOGRAPHY A | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Chromatography, Liquid/methods* | - |
dc.subject.MESH | Fractionation, Field Flow/methods* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Lipoproteins/blood* | - |
dc.subject.MESH | Lipoproteins/isolation & purification | - |
dc.subject.MESH | Phospholipids/blood* | - |
dc.subject.MESH | Phospholipids/isolation & purification | - |
dc.subject.MESH | Tandem Mass Spectrometry/methods* | - |
dc.title | Profiling of phospholipids in lipoproteins by multiplexed hollow fiber flow field-flow fractionation and nanoflow liquid chromatography-tandem mass spectrometry | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Ju Yong Lee | - |
dc.contributor.googleauthor | Hye Kyeong Min | - |
dc.contributor.googleauthor | Donghoon Choi | - |
dc.contributor.googleauthor | Myeong Hee Moon | - |
dc.identifier.doi | 10.1016/j.chroma.2010.01.006 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A04053 | - |
dc.relation.journalcode | J01313 | - |
dc.identifier.eissn | 1873-3778 | - |
dc.identifier.pmid | 20102765 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0021967310000336 | - |
dc.subject.keyword | Hollow fiber flow field-flow fractionation | - |
dc.subject.keyword | Multiplexed HF5 | - |
dc.subject.keyword | Lipoprotein separation | - |
dc.subject.keyword | Phospholipids | - |
dc.subject.keyword | nLC–ESI-MS–MS | - |
dc.subject.keyword | HDL | - |
dc.subject.keyword | LDL | - |
dc.contributor.alternativeName | Choi, Dong Hoon | - |
dc.contributor.affiliatedAuthor | Choi, Dong Hoon | - |
dc.citation.volume | 1217 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 1660 | - |
dc.citation.endPage | 1666 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CHROMATOGRAPHY A, Vol.1217(10) : 1660-1666, 2010 | - |
dc.identifier.rimsid | 37709 | - |
dc.type.rims | ART | - |
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