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Allergenicity of recombinant troponin C from Tyrophagus putrescentiae

 Jeong K.Y.  ;  Kim C.  ;  Un S  ;  Yi M.  ;  Lee I.-Y.  ;  Park J.W.  ;  Hong C.-S.  ;  Yong T.-S. 
 International Archives of Allergy and Immunology, Vol.151(3) : 207-213, 2010 
Journal Title
 International Archives of Allergy and Immunology 
Issue Date
Acaridae/genetics ; Acaridae/immunology* ; Adolescent ; Adult ; Aged ; Allergens/immunology* ; Amino Acid Sequence ; Animals ; Antigens, Plant ; Base Sequence ; Child ; Cross Reactions/immunology ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; Immunoglobulin E/immunology ; Male ; Middle Aged ; Molecular Sequence Data ; Recombinant Proteins/genetics ; Recombinant Proteins/immunology* ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Homology, Amino Acid ; Troponin C/genetics ; Troponin C/immunology* ; Young Adult
Allergen ; Troponin C ; Tyrophagus putrescentiae
Background: The storage mite, Tyrophagus putrescentiae, produces potent allergens, many of which have not been characterized. This study was undertaken to characterize the allergenicity of troponin C from T. putrescentiae.Methods: A cDNA encoding 17.7 kDa troponin C, with homology to cockroach allergen Bla g 6, was identified from T. putrescentiae-expressed sequence tags. Recombinant troponin C was expressed and IgE responses to the recombinant protein were assessed in the presence and absence of 10 mM CaCl(2). Cross-reactivity between T. putrescentiae troponin C and Bla g 6 was tested using an inhibition ELISA. Results: Recombinant T. putrescentiae troponin C shares 62.7-85.5% homology with troponin C from various arthropods. Sera from 5 of 47 subjects in our study group (10.6%) showed IgE binding to the recombinant protein. Interestingly, addition of 10 mM CaCl(2) increased the intensity of IgE binding approximately 2-fold. In an immune-inhibition ELISA with these sera, T. putrescetiae troponin C and Bla g 6 did not cross-react significantly. Conclusions: Troponin C is a new mite allergen with calcium-dependent IgE reactivity.
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Appears in Collections:
5. Research Institutes (연구소) > Institute of Allergy (알레르기연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chung Ryul(김충렬)
Park, Jung Won(박중원) ORCID logo https://orcid.org/0000-0003-0249-8749
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
Yi, Myung Hee(이명희) ORCID logo https://orcid.org/0000-0001-9537-5726
Lee, In Yong(이인용)
Jeong, Kyoung Yong(정경용) ORCID logo https://orcid.org/0000-0001-9887-1426
Hong, Chein Soo(홍천수)
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