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Identification of end-binding 1 (EB1) interacting proteins in Giardia lamblia

Authors
 Kyungpil Kang  ;  Juri Kim  ;  Tai-Soon Yong  ;  Soon-Jung Park 
Citation
 PARASITOLOGY RESEARCH, Vol.106(3) : 723-728, 2010 
Journal Title
 PARASITOLOGY RESEARCH 
ISSN
 0932-0113 
Issue Date
2010
MeSH
Animals ; Cytoskeletal Proteins/isolation & purification ; Cytoskeletal Proteins/metabolism ; Cytoskeleton/chemistry ; Giardia lamblia/chemistry ; Giardia lamblia/physiology* ; Immunoprecipitation ; Microscopy, Fluorescence ; Microtubule-Associated Proteins/metabolism* ; Protein Binding ; Protein Interaction Mapping* ; Protozoan Proteins/isolation & purification ; Protozoan Proteins/metabolism* ; Two-Hybrid System Techniques
Keywords
Indicator Plate ; Candidate Protein ; Putative Amino Acid Sequence ; Adhesive Disk ; Dropout Medium
Abstract
Yeast two-hybrid assays using the end-binding 1 (EB1) protein of Giardia lamblia as bait led to the isolation of four independent clones encoding gamma-giardin, a neurogenic Notch-like protein, and two hypothetical proteins. Interactions between EB1 and the isolated proteins were confirmed by co-immunoprecipitation of in vitro-synthesized candidate proteins and EB1. Localization of these isolated proteins in G. lamblia was examined by immunofluorescence assays, indicating that they were predominantly present in cytoskeletal structures of G. lamblia. These results suggest that EB1 protein functions via interaction with the cytoskeletal components of G. lamblia.
Full Text
http://link.springer.com/article/10.1007%2Fs00436-009-1687-3
DOI
10.1007/s00436-009-1687-3
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Kang, Kyung Pil(강경필)
Kim, Ju Ri(김주리) ORCID logo https://orcid.org/0000-0001-8270-7584
Park, Soon Jung(박순정) ORCID logo https://orcid.org/0000-0002-0423-1944
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/100540
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