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우유단백 항원의 열처리 및 가수분해에 따른 단백의 분포와 항원성 변화

Other Titles
 The effect of heat treatment or hydrolysis on cow’s milk protein distributions and antigenicities 
Authors
 이희선  ;  김미나  ;  홍정연  ;  허원일  ;  김경원  ;  손명현  ;  김규언  ;  이경은  ;  박중원 
Citation
 Allergy Asthma & Respiratory Disease, Vol.2(4) : 259-265, 2014 
Journal Title
 Allergy Asthma & Respiratory Disease 
ISSN
 2288-0402 
Issue Date
2014
Abstract
Purpose: Cow`s milk protein is one of the most common and strongest food allergen. We investigated the effects of heat treatment on the distribution and antigenicities of major allergens from cow`s milk. We also compared the protein distribution and antigenicities among cow`s milk formula and its substitutes. Methods: We heated α-casen, β-lactoglobulin (BLG), α-lactalbumin (ALA), and crude extract of cow`s milk in 100°C boiling water for 1 hour. We prepared crude extracts from cow`s milk formula, partially hydrolyzed milk formula (pHF) and extensively hydrolyzed milk formula (eHF). The protein compositions of all the samples were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The antigenicities were determined by IgE immunoblotting with pooled serum collected from 11 patients with milk allergy. Results: After heating, no significant alteration was found in casein, and the aggregates of ALA and BLG were detected with molecular weights of about 30 and 45 kDa, respectively. The antigenicities of newly detected aggregates were increased. The new aggregates of BLG with increased antigenicities were also found in heated milk total protein. Major milk allergens were not found in pHF, and residual components with a molecular weight below 10 KDa did not show IgE-binding activity. We failed to observe the residual components and antigenicities of eHF. Conclusion: Changes in protein distribution and antigenicity of milk total protein induced by heat treatment may not be significantly different from those of each major allergen. The residual components of pHF could have little IgE-binding capacity, and there may be few or no antigenic components in eHF.
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
김경원(Kim, Kyung Won) ORCID logo https://orcid.org/0000-0003-4529-6135
김규언(Kim, Kyu Earn)
김미나(Kim, Mina) ORCID logo https://orcid.org/0000-0002-1675-0688
박중원(Park, Jung Won) ORCID logo https://orcid.org/0000-0003-0249-8749
손명현(Sohn, Myung Hyun) ORCID logo https://orcid.org/0000-0002-2478-487X
이경은(Lee, Kyung Eun)
이희선(Lee, Hee Seon)
허원일(Heo, Won Il)
홍정연(Hong, Jung Yeon) ORCID logo https://orcid.org/0000-0003-0406-9956
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/100210
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