Cited 6 times in
DA-6034-Induced Mucin Secretion Via Ca2+-Dependent Pathways Through P2Y Receptor Stimulation
DC Field | Value | Language |
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dc.contributor.author | 김태임 | - |
dc.contributor.author | 신동민 | - |
dc.contributor.author | 이훈 | - |
dc.contributor.author | 김응권 | - |
dc.date.accessioned | 2015-01-06T17:32:46Z | - |
dc.date.available | 2015-01-06T17:32:46Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 0146-0404 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/100180 | - |
dc.description.abstract | PURPOSE: We evaluated whether DA-6034 is involved in mucin secretion via P2Y receptor activation and/or intracellular Ca(2+) concentration ([Ca(2+)]i) change. Also, we investigated the effect of P2Y receptor inhibitors or Ca(2+) chelators on the DA-6034-induced mucin secretion and [Ca(2+)]i increases. METHODS: Effects of DA-6034 on mucin expression in primary, cultured, conjunctival epithelial cells was studied using RT-PCR, Western blot analysis, and periodic acid-schiff (PAS) staining. To evaluate thin film layer thickness generated by mucin and fluid secretion, cells were incubated in DA-6034 with/without P2Y antagonists or extracellular/intracellular Ca(2+) chelators, and were imaged with confocal microscope using Texas Red-dextran dye. In addition, DA-6034-induced Ca(2+)-dependent Cl(-) channels opening was evaluated using perforated patch clamp. Fluo-4/AM was used to measure changes in [Ca(2+)]i induced by DA-6034 in Ca(2+)-free or Ca(2+)-containing buffered condition, as well as P2Y antagonists. RESULTS: DA-6034 induced the expression of mucin genes, production of mucin protein, and increase of number of mucin-secreting cells. P2Y antagonists inhibited DA-6034-induced mucin and fluid secretion, which was also affected by extracellular/intracellular Ca(2+) chelators. DA-6034 stimulated Cl(-) channel opening and [Ca(2+)]i elevation. Further, [Ca(2+)]i increases induced by DA-6034 were lacking in either P2Y antagonists or Ca(2+)-free buffered condition, and diminished when endoplasmic reticulum Ca(2+) was depleted by cyclopiazonic acid in Ca(2+)-free buffered condition. CONCLUSIONS: This study demonstrated that DA-6034 has a potential to induce mucin secretion via Ca(2+)-dependent pathways through P2Y receptors in multilayer, cultured, human conjunctival epithelial cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 6565~6574 | - |
dc.relation.isPartOf | INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adult | - |
dc.subject.MESH | Blotting, Western | - |
dc.subject.MESH | Calcium/metabolism* | - |
dc.subject.MESH | Calcium Signaling | - |
dc.subject.MESH | Cell Proliferation | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Conjunctiva/cytology | - |
dc.subject.MESH | Conjunctiva/metabolism | - |
dc.subject.MESH | Epithelial Cells/cytology | - |
dc.subject.MESH | Epithelial Cells/metabolism | - |
dc.subject.MESH | Flavonoids/pharmacology* | - |
dc.subject.MESH | Gene Expression Regulation/drug effects | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Intracellular Fluid/metabolism* | - |
dc.subject.MESH | Membrane Potentials | - |
dc.subject.MESH | Middle Aged | - |
dc.subject.MESH | Mucins/drug effects | - |
dc.subject.MESH | Mucins/genetics | - |
dc.subject.MESH | Mucins/secretion* | - |
dc.subject.MESH | Patch-Clamp Techniques | - |
dc.subject.MESH | RNA/genetics | - |
dc.subject.MESH | Receptors, Purinergic P2Y/drug effects* | - |
dc.subject.MESH | Receptors, Purinergic P2Y/metabolism | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.subject.MESH | Signal Transduction | - |
dc.title | DA-6034-Induced Mucin Secretion Via Ca2+-Dependent Pathways Through P2Y Receptor Stimulation | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Ophthalmology (안과학) | - |
dc.contributor.googleauthor | Hun Lee | - |
dc.contributor.googleauthor | Eung Kweon Kim | - |
dc.contributor.googleauthor | Ji Yeon Kim | - |
dc.contributor.googleauthor | Yu-Mi Yang | - |
dc.contributor.googleauthor | Dong Min Shin | - |
dc.contributor.googleauthor | Kyung Koo Kang | - |
dc.contributor.googleauthor | Tae-im Kim | - |
dc.identifier.doi | 10.1167/iovs.14-13875 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01080 | - |
dc.contributor.localId | A02091 | - |
dc.contributor.localId | A03344 | - |
dc.contributor.localId | A00831 | - |
dc.relation.journalcode | J01187 | - |
dc.identifier.eissn | 1552-5783 | - |
dc.identifier.pmid | 25212776 | - |
dc.identifier.url | http://www.iovs.org/content/55/10/6565.long | - |
dc.subject.keyword | DA-6034 | - |
dc.subject.keyword | calcium signaling | - |
dc.subject.keyword | cultured conjunctival epithelial cells | - |
dc.subject.keyword | mucins | - |
dc.subject.keyword | purinergic receptors | - |
dc.contributor.alternativeName | Kim, Tae Im | - |
dc.contributor.alternativeName | Shin, Dong Min | - |
dc.contributor.alternativeName | Lee, Hun | - |
dc.contributor.alternativeName | Kim, Eung Kweon | - |
dc.contributor.affiliatedAuthor | Kim, Tae Im | - |
dc.contributor.affiliatedAuthor | Shin, Dong Min | - |
dc.contributor.affiliatedAuthor | Lee, Hun | - |
dc.contributor.affiliatedAuthor | Kim, Eung Kweon | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 55 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 6565 | - |
dc.citation.endPage | 6574 | - |
dc.identifier.bibliographicCitation | INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, Vol.55(10) : 6565-6574, 2014 | - |
dc.identifier.rimsid | 51732 | - |
dc.type.rims | ART | - |
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