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DA-6034-Induced Mucin Secretion Via Ca2+-Dependent Pathways Through P2Y Receptor Stimulation

DC FieldValueLanguage
dc.contributor.author김태임-
dc.contributor.author신동민-
dc.contributor.author이훈-
dc.contributor.author김응권-
dc.date.accessioned2015-01-06T17:32:46Z-
dc.date.available2015-01-06T17:32:46Z-
dc.date.issued2014-
dc.identifier.issn0146-0404-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/100180-
dc.description.abstractPURPOSE: We evaluated whether DA-6034 is involved in mucin secretion via P2Y receptor activation and/or intracellular Ca(2+) concentration ([Ca(2+)]i) change. Also, we investigated the effect of P2Y receptor inhibitors or Ca(2+) chelators on the DA-6034-induced mucin secretion and [Ca(2+)]i increases. METHODS: Effects of DA-6034 on mucin expression in primary, cultured, conjunctival epithelial cells was studied using RT-PCR, Western blot analysis, and periodic acid-schiff (PAS) staining. To evaluate thin film layer thickness generated by mucin and fluid secretion, cells were incubated in DA-6034 with/without P2Y antagonists or extracellular/intracellular Ca(2+) chelators, and were imaged with confocal microscope using Texas Red-dextran dye. In addition, DA-6034-induced Ca(2+)-dependent Cl(-) channels opening was evaluated using perforated patch clamp. Fluo-4/AM was used to measure changes in [Ca(2+)]i induced by DA-6034 in Ca(2+)-free or Ca(2+)-containing buffered condition, as well as P2Y antagonists. RESULTS: DA-6034 induced the expression of mucin genes, production of mucin protein, and increase of number of mucin-secreting cells. P2Y antagonists inhibited DA-6034-induced mucin and fluid secretion, which was also affected by extracellular/intracellular Ca(2+) chelators. DA-6034 stimulated Cl(-) channel opening and [Ca(2+)]i elevation. Further, [Ca(2+)]i increases induced by DA-6034 were lacking in either P2Y antagonists or Ca(2+)-free buffered condition, and diminished when endoplasmic reticulum Ca(2+) was depleted by cyclopiazonic acid in Ca(2+)-free buffered condition. CONCLUSIONS: This study demonstrated that DA-6034 has a potential to induce mucin secretion via Ca(2+)-dependent pathways through P2Y receptors in multilayer, cultured, human conjunctival epithelial cells.-
dc.description.statementOfResponsibilityopen-
dc.format.extent6565~6574-
dc.relation.isPartOfINVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdult-
dc.subject.MESHBlotting, Western-
dc.subject.MESHCalcium/metabolism*-
dc.subject.MESHCalcium Signaling-
dc.subject.MESHCell Proliferation-
dc.subject.MESHCells, Cultured-
dc.subject.MESHConjunctiva/cytology-
dc.subject.MESHConjunctiva/metabolism-
dc.subject.MESHEpithelial Cells/cytology-
dc.subject.MESHEpithelial Cells/metabolism-
dc.subject.MESHFlavonoids/pharmacology*-
dc.subject.MESHGene Expression Regulation/drug effects-
dc.subject.MESHHumans-
dc.subject.MESHIntracellular Fluid/metabolism*-
dc.subject.MESHMembrane Potentials-
dc.subject.MESHMiddle Aged-
dc.subject.MESHMucins/drug effects-
dc.subject.MESHMucins/genetics-
dc.subject.MESHMucins/secretion*-
dc.subject.MESHPatch-Clamp Techniques-
dc.subject.MESHRNA/genetics-
dc.subject.MESHReceptors, Purinergic P2Y/drug effects*-
dc.subject.MESHReceptors, Purinergic P2Y/metabolism-
dc.subject.MESHReverse Transcriptase Polymerase Chain Reaction-
dc.subject.MESHSignal Transduction-
dc.titleDA-6034-Induced Mucin Secretion Via Ca2+-Dependent Pathways Through P2Y Receptor Stimulation-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Ophthalmology (안과학)-
dc.contributor.googleauthorHun Lee-
dc.contributor.googleauthorEung Kweon Kim-
dc.contributor.googleauthorJi Yeon Kim-
dc.contributor.googleauthorYu-Mi Yang-
dc.contributor.googleauthorDong Min Shin-
dc.contributor.googleauthorKyung Koo Kang-
dc.contributor.googleauthorTae-im Kim-
dc.identifier.doi10.1167/iovs.14-13875-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01080-
dc.contributor.localIdA02091-
dc.contributor.localIdA03344-
dc.contributor.localIdA00831-
dc.relation.journalcodeJ01187-
dc.identifier.eissn1552-5783-
dc.identifier.pmid25212776-
dc.identifier.urlhttp://www.iovs.org/content/55/10/6565.long-
dc.subject.keywordDA-6034-
dc.subject.keywordcalcium signaling-
dc.subject.keywordcultured conjunctival epithelial cells-
dc.subject.keywordmucins-
dc.subject.keywordpurinergic receptors-
dc.contributor.alternativeNameKim, Tae Im-
dc.contributor.alternativeNameShin, Dong Min-
dc.contributor.alternativeNameLee, Hun-
dc.contributor.alternativeNameKim, Eung Kweon-
dc.contributor.affiliatedAuthorKim, Tae Im-
dc.contributor.affiliatedAuthorShin, Dong Min-
dc.contributor.affiliatedAuthorLee, Hun-
dc.contributor.affiliatedAuthorKim, Eung Kweon-
dc.rights.accessRightsfree-
dc.citation.volume55-
dc.citation.number10-
dc.citation.startPage6565-
dc.citation.endPage6574-
dc.identifier.bibliographicCitationINVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, Vol.55(10) : 6565-6574, 2014-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers

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