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PI3K/Akt pathway regulates retinoic acid-induced Hox gene expression in F9 cells

DC Field Value Language
dc.contributor.author김명희-
dc.contributor.author이유라-
dc.contributor.author이지연-
dc.date.accessioned2015-01-06T17:16:19Z-
dc.date.available2015-01-06T17:16:19Z-
dc.date.issued2014-
dc.identifier.issn0012-1592-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/99663-
dc.description.abstractRetinoic acid (RA), the most potent natural form of vitamin A, is a key morphogen in vertebrate development and a potent regulator of both adult and embryonic cell differentiation. Specifically, RA regulates clustered Hox gene expression during embryogenesis and is required to establish the anteroposterior body plan. The PI3K/Akt pathway was also reported to play an essential role in the process of RA-induced cell differentiation. Therefore, we tested whether the PI3K/Akt pathway is involved in RA-induced Hox gene expression in a F9 murine embryonic teratocarcinoma cells. To examine the effect of PI3K/Akt signaling on RA-induced initiation of collinear expression of Hox genes, F9 cells were treated with RA in the presence or absence of PI3K inhibitor LY294002, and time-course gene expression profiles for all 39 Hox genes located in four different clusters—Hoxa, Hoxb, Hoxc, and Hoxd—were analyzed. Collinear expression of Hoxa and -b cluster genes was initiated earlier than that of the -c and -d clusters upon RA treatment. When LY294002 was applied along with RA, collinear expression induced by RA was delayed, suggesting that the PI3K/Akt signaling pathway somehow regulates RA-induced collinear expression of Hox genes in F9 cells. The initiation of Hox collinear expression by RA and the delayed expression following LY294002 in F9 cells would provide a good model system to decipher the yet to be answered de novo collinear expression of Hox genes during gastrulation, which make the gastrulating cells to remember their positional address along the AP body axis in the developing embryo.-
dc.description.statementOfResponsibilityopen-
dc.format.extent518~525-
dc.relation.isPartOfDEVELOPMENT GROWTH & DIFFERENTIATION-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHBlotting, Western-
dc.subject.MESHChromones/pharmacology-
dc.subject.MESHEmbryonal Carcinoma Stem Cells/metabolism*-
dc.subject.MESHGastrulation/genetics-
dc.subject.MESHGastrulation/physiology*-
dc.subject.MESHGene Expression Regulation, Developmental/drug effects*-
dc.subject.MESHGene Expression Regulation, Developmental/physiology-
dc.subject.MESHGenes, Homeobox/physiology*-
dc.subject.MESHMice-
dc.subject.MESHMorpholines/pharmacology-
dc.subject.MESHPhosphatidylinositol 3-Kinases/antagonists & inhibitors-
dc.subject.MESHPhosphatidylinositol 3-Kinases/metabolism-
dc.subject.MESHProto-Oncogene Proteins c-akt/metabolism-
dc.subject.MESHReverse Transcriptase Polymerase Chain Reaction-
dc.subject.MESHSignal Transduction/genetics-
dc.subject.MESHSignal Transduction/physiology*-
dc.subject.MESHTranscriptome-
dc.subject.MESHTretinoin/metabolism-
dc.subject.MESHTretinoin/pharmacology*-
dc.titlePI3K/Akt pathway regulates retinoic acid-induced Hox gene expression in F9 cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Anatomy (해부학)-
dc.contributor.googleauthorYoura Lee-
dc.contributor.googleauthorJi-Yeon Lee-
dc.contributor.googleauthorMyoung Hee Kim-
dc.identifier.doi10.1111/dgd.12152-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00432-
dc.contributor.localIdA03011-
dc.contributor.localIdA03194-
dc.relation.journalcodeJ00712-
dc.identifier.eissn1440-169X-
dc.identifier.pmid25212816-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1111/dgd.12152/abstract-
dc.subject.keywordPI3K/Akt signal-
dc.subject.keywordRetinoic acid-
dc.subject.keywordanteroposterior axis-
dc.subject.keywordcollinear expression of Hox genes-
dc.subject.keyworddevelopment-
dc.contributor.alternativeNameKim, Myoung Hee-
dc.contributor.alternativeNameLee, You Ra-
dc.contributor.alternativeNameLee, Ji Yeon-
dc.contributor.affiliatedAuthorKim, Myoung Hee-
dc.contributor.affiliatedAuthorLee, You Ra-
dc.contributor.affiliatedAuthorLee, Ji Yeon-
dc.rights.accessRightsfree-
dc.citation.volume56-
dc.citation.number7-
dc.citation.startPage518-
dc.citation.endPage525-
dc.identifier.bibliographicCitationDEVELOPMENT GROWTH & DIFFERENTIATION, Vol.56(7) : 518-525, 2014-
dc.identifier.rimsid57038-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers

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