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Knockdown of TWIST1 enhances arsenic trioxide- and ionizing radiation-induced cell death in lung cancer cells by promoting mitochondrial dysfunction

DC FieldValueLanguage
dc.contributor.author이윤한-
dc.contributor.author이현규-
dc.date.accessioned2015-01-06T17:03:22Z-
dc.date.available2015-01-06T17:03:22Z-
dc.date.issued2014-
dc.identifier.issn0006-291X-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/99272-
dc.description.abstractTWIST1 is implicated in the process of epithelial mesenchymal transition, metastasis, stemness, and drug resistance in cancer cells, and therefore is a potential target for cancer therapy. In the present study, we found that knockdown of TWIST1 by small interfering RNA (siRNA) enhanced arsenic trioxide (ATO)- and ionizing radiation (IR)-induced cell death in non-small-cell lung cancer cells. Interestingly, intracellular reactive oxygen species levels were increased in cells treated with TWIST1 siRNA and further increased by co-treatment with ATO or IR. Pretreatment of lung cancer cells with the antioxidant N-acetyl-cysteine markedly suppressed the cell death induced by combined treatment with TWIST1 siRNA and ATO or IR. Moreover, treatment of cells with TWIST1 siRNA induced mitochondrial membrane depolarization and significantly increased mitochondrial fragmentation (fission) and upregulated the fission-related proteins FIS1 and DRP1. Collectively, our results demonstrate that siRNA-mediated TWIST1 knockdown induces mitochondrial dysfunction and enhances IR- and ATO-induced cell death in lung cancer cells.-
dc.description.statementOfResponsibilityopen-
dc.format.extent490~495-
dc.relation.isPartOfBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAcetylcysteine/pharmacology-
dc.subject.MESHArsenicals-
dc.subject.MESHCarcinoma, Non-Small-Cell Lung/metabolism-
dc.subject.MESHCarcinoma, Non-Small-Cell Lung/pathology-
dc.subject.MESHCarcinoma, Non-Small-Cell Lung/radiotherapy-
dc.subject.MESHCell Death/drug effects-
dc.subject.MESHCell Death/radiation effects-
dc.subject.MESHGene Knockdown Techniques-
dc.subject.MESHHumans-
dc.subject.MESHMembrane Potential, Mitochondrial/drug effects-
dc.subject.MESHNuclear Proteins/biosynthesis-
dc.subject.MESHNuclear Proteins/genetics*-
dc.subject.MESHOxides-
dc.subject.MESHRNA, Small Interfering/pharmacology-
dc.subject.MESHRadiation, Ionizing-
dc.subject.MESHReactive Oxygen Species/metabolism-
dc.subject.MESHTwist-Related Protein 1/biosynthesis-
dc.subject.MESHTwist-Related Protein 1/genetics*-
dc.titleKnockdown of TWIST1 enhances arsenic trioxide- and ionizing radiation-induced cell death in lung cancer cells by promoting mitochondrial dysfunction-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Radiation Oncology (방사선종양학)-
dc.contributor.googleauthorSung-Keum Seo-
dc.contributor.googleauthorJae-Hee Kim-
dc.contributor.googleauthorHa-Na Choi-
dc.contributor.googleauthorTae-Boo Choe-
dc.contributor.googleauthorSeok-Il Hong-
dc.contributor.googleauthorJae-Youn Yi-
dc.contributor.googleauthorSang-Gu Hwang-
dc.contributor.googleauthorHyun-Gyu Lee-
dc.contributor.googleauthorYun-Han Lee-
dc.contributor.googleauthorIn-Chul Park-
dc.identifier.doi10.1016/j.bbrc.2014.05.030-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA03029-
dc.contributor.localIdA03289-
dc.relation.journalcodeJ00281-
dc.identifier.eissn1090-2104-
dc.identifier.pmid24845567-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0006291X14008845-
dc.subject.keywordArsenic trioxide-
dc.subject.keywordIrradiation-
dc.subject.keywordMitochondria dysfunction-
dc.subject.keywordReactive oxygen species-
dc.subject.keywordTWIST1-
dc.contributor.alternativeNameLee, Yun Han-
dc.contributor.alternativeNameLee, Hyun Gyu-
dc.contributor.affiliatedAuthorLee, Yun Han-
dc.contributor.affiliatedAuthorLee, Hyun Gyu-
dc.rights.accessRightsfree-
dc.citation.volume449-
dc.citation.number4-
dc.citation.startPage490-
dc.citation.endPage495-
dc.identifier.bibliographicCitationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.449(4) : 490-495, 2014-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers

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