Cited 49 times in
The Molecular Mechanism Underlying the Proliferating and Preconditioning Effect of Vitamin C on Adipose-Derived Stem Cells
DC Field | Value | Language |
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dc.contributor.author | 송승용 | - |
dc.date.accessioned | 2015-01-06T16:59:15Z | - |
dc.date.available | 2015-01-06T16:59:15Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 1547-3287 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/99154 | - |
dc.description.abstract | Although adipose-derived stem cells (ASCs) show promise for cell therapy, there is a tremendous need for developing ASC activators. In the present study, we investigated whether or not vitamin C increases the survival, proliferation, and hair-regenerative potential of ASCs. In addition, we tried to find the molecular mechanisms underlying the vitamin C-mediated stimulation of ASCs. Sodium-dependent vitamin C transporter 2 (SVCT2) is expressed in ASCs, and mediates uptake of vitamin C into ASCs. Vitamin C increased the survival and proliferation of ASCs in a dose-dependent manner. Vitamin C increased ERK1/2 phosphorylation, and inhibition of the mitogen-activated protein kinase (MAPK) pathway attenuated the proliferation of ASCs. Microarray and quantitative polymerase chain reaction showed that vitamin C primarily upregulated expression of proliferation-related genes, including Fos, E2F2, Ier2, Mybl1, Cdc45, JunB, FosB, and Cdca5, whereas Fos knock-down using siRNA significantly decreased vitamin C-mediated ASC proliferation. In addition, vitamin C-treated ASCs accelerated the telogen-to-anagen transition in C3H/HeN mice, and conditioned medium from vitamin C-treated ASCs increased the hair length and the Ki67-positive matrix keratinocytes in hair organ culture. Vitamin C increased the mRNA expression of HGF, IGFBP6, VEGF, bFGF, and KGF, which may mediate hair growth promotion. In summary, vitamin C is transported via SVCT2, and increased ASC proliferation is mediated by the MAPK pathway. In addition, vitamin C preconditioning enhanced the hair growth promoting effect of ASCs. Because vitamin C is safe and effective, it could be used to increase the yield and regenerative potential of ASCs. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1364~1376 | - |
dc.relation.isPartOf | STEM CELLS AND DEVELOPMENT | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adipocytes/cytology* | - |
dc.subject.MESH | Adipocytes/drug effects | - |
dc.subject.MESH | Adipose Tissue/cytology* | - |
dc.subject.MESH | Adipose Tissue/drug effects | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Ascorbic Acid/administration & dosage* | - |
dc.subject.MESH | Cell Differentiation/drug effects | - |
dc.subject.MESH | Cell Proliferation/genetics | - |
dc.subject.MESH | Hair/drug effects | - |
dc.subject.MESH | Hair/growth & development* | - |
dc.subject.MESH | Keratinocytes/drug effects | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Protein Biosynthesis | - |
dc.subject.MESH | Sodium-Coupled Vitamin C Transporters/biosynthesis | - |
dc.subject.MESH | Stem Cells/cytology | - |
dc.subject.MESH | Stem Cells/drug effects | - |
dc.title | The Molecular Mechanism Underlying the Proliferating and Preconditioning Effect of Vitamin C on Adipose-Derived Stem Cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Plastic Surgery & Reconstructive Surgery (성형외과학) | - |
dc.contributor.googleauthor | Kim Ji Hye | - |
dc.contributor.googleauthor | Kim Wang-Kyun | - |
dc.contributor.googleauthor | Sung Young Kwan | - |
dc.contributor.googleauthor | Kwack Mi Hee | - |
dc.contributor.googleauthor | Song Seung Yong | - |
dc.contributor.googleauthor | Choi Joon-Seok | - |
dc.contributor.googleauthor | Park Sang Gyu | - |
dc.contributor.googleauthor | Yi TacGhee | - |
dc.contributor.googleauthor | Lee Hyun-Joo | - |
dc.contributor.googleauthor | Kim Dae-Duk | - |
dc.contributor.googleauthor | Seo Hyun Min | - |
dc.contributor.googleauthor | Song Sun U | - |
dc.contributor.googleauthor | Sung Jong-Hyuk | - |
dc.identifier.doi | 10.1089/scd.2013.0460 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02032 | - |
dc.relation.journalcode | J02684 | - |
dc.identifier.eissn | 1557-8534 | - |
dc.identifier.pmid | 24524758 | - |
dc.identifier.url | http://online.liebertpub.com/doi/abs/10.1089/scd.2013.0460 | - |
dc.contributor.alternativeName | Song, Seung Yong | - |
dc.contributor.affiliatedAuthor | Song, Seung Yong | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 23 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 1364 | - |
dc.citation.endPage | 1376 | - |
dc.identifier.bibliographicCitation | STEM CELLS AND DEVELOPMENT, Vol.23(12) : 1364-1376, 2014 | - |
dc.identifier.rimsid | 51184 | - |
dc.type.rims | ART | - |
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