0 627

Cited 10 times in

Loop-mediated isothermal amplification of vanA gene enables a rapid and naked-eye detection of vancomycin-resistant enterococci infection

DC Field Value Language
dc.contributor.author이경원-
dc.contributor.author이재면-
dc.contributor.author박전한-
dc.contributor.author용동은-
dc.contributor.author윤상선-
dc.date.accessioned2015-01-06T16:55:46Z-
dc.date.available2015-01-06T16:55:46Z-
dc.date.issued2014-
dc.identifier.issn0167-7012-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/99043-
dc.description.abstractVancomycin-resistant enterococci (VRE) are one of the leading causes of nosocomial infection at intensive care unit (ICU). A rapid and sensitive detection of VRE infection is in high demand for timely and suitable antibiotic treatment. Here, we optimized a distinct DNA-based diagnostic technique, loop-mediated isothermal amplification (LAMP) for a rapid detection of the presence of vanA gene, a critical component of the gene cluster required for vancomycin resistance. Amplification efficiency was optimal at 62 °C and with 2 mM MgSO4. The detection limit of the DNA template was 80 pg and LAMP amplicons were detected within 40 min; thereby suggesting a potential applicability of LAMP as a sensitive and urgent diagnostic method. Furthermore, positive LAMP reaction was directly detected with the naked-eye by monitoring the formation of a white precipitate or the color change induced by hydroxy naphthol blue (HNB) dye. Finally, 56 clinical isolates were successfully tested for the presence of vanA gene by LAMP, which was determined to be more sensitive than PCR. Together, our results clearly demonstrate the usefulness of LAMP for the diagnosis of VRE infection.-
dc.description.statementOfResponsibilityopen-
dc.format.extent61~66-
dc.relation.isPartOfJOURNAL OF MICROBIOLOGICAL METHODS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHBacterial Proteins/genetics*-
dc.subject.MESHCarbon-Oxygen Ligases/genetics*-
dc.subject.MESHCross Infection/microbiology*-
dc.subject.MESHFeces/microbiology-
dc.subject.MESHGram-Positive Bacterial Infections/microbiology*-
dc.subject.MESHHumans-
dc.subject.MESHNucleic Acid Amplification Techniques/methods*-
dc.subject.MESHVancomycin-Resistant Enterococci/enzymology*-
dc.subject.MESHVancomycin-Resistant Enterococci/genetics-
dc.subject.MESHVancomycin-Resistant Enterococci/isolation & purification*-
dc.titleLoop-mediated isothermal amplification of vanA gene enables a rapid and naked-eye detection of vancomycin-resistant enterococci infection-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Laboratory Medicine (진단검사의학)-
dc.contributor.googleauthorHye Jin Kim-
dc.contributor.googleauthorYu Jin Kim-
dc.contributor.googleauthorDong Eun Yong-
dc.contributor.googleauthorKyungwon Lee-
dc.contributor.googleauthorJeon Han Park-
dc.contributor.googleauthorJae Myun Lee-
dc.contributor.googleauthorSang Sun Yoon-
dc.identifier.doi10.1016/j.mimet.2014.05.021-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02649-
dc.contributor.localIdA03071-
dc.contributor.localIdA01641-
dc.contributor.localIdA02423-
dc.contributor.localIdA02558-
dc.relation.journalcodeJ01592-
dc.identifier.eissn1872-8359-
dc.identifier.pmid24925601-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0167701214001511-
dc.subject.keywordDiagnosis-
dc.subject.keywordLoop-mediated isothermal amplification (LAMP)-
dc.subject.keywordVancomycin-resistant enterococci (VRE)-
dc.contributor.alternativeNameLee, Kyung Won-
dc.contributor.alternativeNameLee, Jae Myun-
dc.contributor.alternativeNamePark, Jeon Han-
dc.contributor.alternativeNameYong, Dong Eun-
dc.contributor.alternativeNameYoon, Sang Sun-
dc.contributor.affiliatedAuthorLee, Kyung Won-
dc.contributor.affiliatedAuthorLee, Jae Myun-
dc.contributor.affiliatedAuthorPark, Jeon Han-
dc.contributor.affiliatedAuthorYong, Dong Eun-
dc.contributor.affiliatedAuthorYoon, Sang Sun-
dc.rights.accessRightsfree-
dc.citation.volume104C-
dc.citation.startPage61-
dc.citation.endPage66-
dc.identifier.bibliographicCitationJOURNAL OF MICROBIOLOGICAL METHODS, Vol.104C : 61-66, 2014-
dc.identifier.rimsid50228-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.