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Cited 4 times in

Production of Egg Yolk Antibodies Specific to House Dust Mite Proteins

DC FieldValueLanguage
dc.contributor.author허원일-
dc.contributor.author홍정연-
dc.contributor.author김규언-
dc.contributor.author김미나-
dc.contributor.author손명현-
dc.contributor.author이경은-
dc.contributor.author김경원-
dc.date.accessioned2015-01-06T16:54:49Z-
dc.date.available2015-01-06T16:54:49Z-
dc.date.issued2014-
dc.identifier.issn0513-5796-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/99012-
dc.description.abstractPURPOSE: House dust mites (HDMs) are an important source of indoor allergens associated with asthma, rhinitis and atopic dermatitis. Chicken immunoglobulin (Ig) Y is known to be a good alternative to mice and rabbit antibody production. In this study, we produced IgYs specific to HDMs and investigated their IgE immunoreactivities. MATERIALS AND METHODS: Total IgYs were isolated from the yolks of White Leghorn hens immunized with either Dermatophagoides pteronyssinus or D. farinae protein extract. Control antibodies were separated from the yolks of immunized hens with phosphate buffered saline. IgYs specific to HDMs were analyzed using enzyme-linked immunosorbent assay and Western blotting analysis. RESULTS: The concentration of egg IgY specific to D. farinae in an immunized hen increased and the highest achieved was 661.3 ug/mg (per an egg) on day 47, compared with 760 ug/mg IgY specific to D. pteronyssinus on day 16. The D. pteronyssinus or D. farinae-specific IgY was detected by binding of each mite proteins, and their immunoreactivities were elevated dependent of the specific IgY concentration. CONCLUSION: IgY specific to HDMs may be a promising antibody for immunological diagnosis as well as identification of possible resistance relating to HDM allergy.-
dc.description.statementOfResponsibilityopen-
dc.format.extent999~1004-
dc.relation.isPartOfYonsei Medical Journal-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleProduction of Egg Yolk Antibodies Specific to House Dust Mite Proteins-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentYonsei Biomedical Research Center (연세의생명연구원)-
dc.contributor.googleauthorKyung Eun Lee-
dc.contributor.googleauthorBeom Ku Han-
dc.contributor.googleauthorJae Yong Han-
dc.contributor.googleauthorJung Yeon Hong-
dc.contributor.googleauthorMi Na Kim-
dc.contributor.googleauthorWon Il Heo-
dc.contributor.googleauthorMyung Hyun Sohn-
dc.contributor.googleauthorKyung Won Kim-
dc.contributor.googleauthorKyu Earn Kim-
dc.identifier.doi10.3349/ymj.2014.55.4.999-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02651-
dc.contributor.localIdA04361-
dc.contributor.localIdA04431-
dc.contributor.localIdA00327-
dc.contributor.localIdA00441-
dc.contributor.localIdA01967-
dc.contributor.localIdA00303-
dc.relation.journalcodeJ02813-
dc.contributor.alternativeNameHeo, Won Il-
dc.contributor.alternativeNameHong, Jung Yeon-
dc.contributor.alternativeNameKim, Kyu Earn-
dc.contributor.alternativeNameKim, Mina-
dc.contributor.alternativeNameSon, Myung Hyun-
dc.contributor.alternativeNameLee, Kyung Eun-
dc.contributor.alternativeNameKim, Kyung Won-
dc.contributor.affiliatedAuthorLee, Kyung Eun-
dc.contributor.affiliatedAuthorHeo, Won Il-
dc.contributor.affiliatedAuthorHong, Jung Yeon-
dc.contributor.affiliatedAuthorKim, Kyu Earn-
dc.contributor.affiliatedAuthorKim, Mina-
dc.contributor.affiliatedAuthorSon, Myung Hyun-
dc.contributor.affiliatedAuthorKim, Kyung Won-
dc.citation.volume55-
dc.citation.number4-
dc.citation.startPage999-
dc.citation.endPage1004-
dc.identifier.bibliographicCitationYonsei Medical Journal, Vol.55(4) : 999-1004, 2014-
Appears in Collections:
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실) > 1. Journal Papers

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