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Ultrafine particles of Ulmus davidiana var. japonica induce apoptosis of gastric cancer cells via activation of caspase and endoplasmic reticulum stress

Authors
 Joungjwa Ahn  ;  Jong Suk Lee  ;  Kyung Mi Yang 
Citation
 ARCHIVES OF PHARMACAL RESEARCH, Vol.37(6) : 783-792, 2014 
Journal Title
 ARCHIVES OF PHARMACAL RESEARCH 
ISSN
 0253-6269 
Issue Date
2014
MeSH
Apoptosis/drug effects ; Apoptosis/physiology* ; Caspases/metabolism* ; Cell Line, Tumor ; Cell Survival/drug effects ; Cell Survival/physiology ; Dose-Response Relationship, Drug ; Endoplasmic Reticulum Stress/drug effects ; Endoplasmic Reticulum Stress/physiology* ; Enzyme Activation/drug effects ; Enzyme Activation/physiology ; Humans ; Organ Culture Techniques ; Particle Size ; Plant Extracts/isolation & purification ; Plant Extracts/pharmacology* ; Stomach Neoplasms/metabolism* ; Ulmus*
Keywords
Ulmus davidiana var. japonica ; Apoptosis ; ER stress ; Gastric cancer ; Caspase
Abstract
Small-sized particles are more suitable for targeted delivery and are therapeutically more effective than large-sized particles. In this study, we investigated the anticancer effects of ultrafine particles of Ulmus davidiana var. japonica (ufUJ) on human gastric cancer cell lines SNU-1, SNU-216, and SNU-484. ufUJ induced apoptosis by the proteolytic activation of caspase-9, caspase-6, and caspase-3 and cleavage of poly (ADP-ribose) polymerase. The expression levels of the endoplasmic reticulum stress-related protein BiP markedly increased after ufUJ treatment. BiP knockdown decreased ufUJ-induced cell death. ufUJ-induced apoptosis was inhibited by the caspase-3 inhibitor z-DEVD-fmk, caspase-6 inhibitor z-VEID-fmk, and caspase-9 inhibitor z-LEHD-fmk, and by siRNAs against caspases 3, 6, and 9. Gastric cancer cells did not show anchorage-independent growth in the presence of ufUJ. However, cells treated with caspase inhibitors showed an enhanced colony-forming ability. These findings may be helpful in the prevention of gastric cancer and in the development of functional foods.
Files in This Item:
T201401739.pdf Download
DOI
10.1007/s12272-013-0312-2
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Yang, Kyung Mi(양경미)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/98917
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