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Molecular epidemiology of Pseudomonas aeruginosa clinical isolates from Korea producing β-lactamases with extended-spectrum activity

Authors
 Il Kwon Bae  ;  Borum Suh  ;  Seok Hoon Jeong  ;  Kang-Kyun Wang  ;  Yong-Rok Kim  ;  Dongeun Yong  ;  Kyungwon Lee 
Citation
 DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, Vol.79(3) : 373-377, 2014 
Journal Title
 DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE 
ISSN
 0732-8893 
Issue Date
2014
MeSH
Anti-Bacterial Agents/pharmacology ; Drug Resistance, Bacterial ; Genotype ; Hospitals, University ; Humans ; Molecular Epidemiology ; Multilocus Sequence Typing* ; Prevalence ; Pseudomonas Infections/epidemiology* ; Pseudomonas Infections/microbiology* ; Pseudomonas aeruginosa/classification* ; Pseudomonas aeruginosa/enzymology* ; Pseudomonas aeruginosa/genetics ; Pseudomonas aeruginosa/isolation & purification ; Republic of Korea/epidemiology ; beta-Lactamases/genetics* ; beta-Lactams/pharmacology
Keywords
Clonal complex 235 (CC235) ; Extended-spectrum oxacillinase ; Extended-spectrum β-lactamase ; Metallo-β-lactamase
Abstract
This study was performed to investigate the prevalence and molecular epidemiology of Pseudomonas aeruginosa isolates from Korea that produce enzymes with extended-spectrum (ES) activity to β-lactams. A total of 205 non-duplicate P. aeruginosa clinical isolates were collected from 18 university hospitals in Korea. PCR and sequencing experiments were performed to identify genes encoding β-lactamases. PCR mapping and sequencing of the regions surrounding the β-lactamase genes were performed. Multilocus sequence typing experiments were performed. The most common sequence type (ST) was ST235 (n = 96), and 2 single-locus variants of ST235, ST1015 (n = 1) and ST1162 (n = 1), were also identified. These 3 STs were grouped as a clonal complex (CC), CC235. The remaining 107 isolates were identified as 59 different STs. Isolates belonging to CC235 showed higher rates of non-susceptibility to imipenem (85.4% versus 47.7%) and meropenem (92.7% versus 52.3%) compared to non-CC235 isolates. All the metallo-β-lactamase (MBL)–producing isolates were identified as CC235, except for 1 ST591. Genes encoding OXA-17 and OXA-142 were detected in 1 isolate and 4 isolates of CC235, respectively; while the blaSHV-12 gene was detected in 4 non-CC235 isolates. Class A and D β-lactamases with ES activity play a role in acquiring ceftazidime resistance in P. aeruginosa in Korea. Production of IMP-6 and VIM-2 MBLs is the main mechanisms in acquiring resistance to ceftazidime and carbapenems in P. aeruginosa isolates in Korea. Clonal spread of P. aeruginosa CC235 may be an important conduit for the dissemination of MBL genes in Korea.
Full Text
http://www.sciencedirect.com/science/article/pii/S0732889314001175
DOI
10.1016/j.diagmicrobio.2014.03.007
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
Jeong, Seok Hoon(정석훈) ORCID logo https://orcid.org/0000-0001-9290-897X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/98814
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